Effects of tannins in pomegranate bark on endogenous metabolites in rats with renal fibrosis
OBJFCTIVE To investigate the effects of tannins from pomegranate bark on endogenous metabolites in rats with renal fibrosis by using metabonomics method,find potential biomarkers and analyze the metabolizing pathway,and provide relevant theoretical background on the treatment of renal fibrosis and utilization of tannins from pomegranate bark.METHODS SD rats were randomly divided into five groups,i.e.,sham operation group,model group,valsartan group,high dose and low dose groups of tannins from pomegranate bark.Except for sham operation group,left ureter of rat was ligated to construct renal fibrosis model,while in sham operated group,ureter would not be ligated or cut off.In the five groups,urine were collected from rats in Week 3 and analyzed by liquid chromatography-high resolution mass spectrometry (LC-MS) to obtain total ion current.Data from LC-MS were pretreated,and imported into SIMCA-P to perform principal component analysis (PCA) and orthogonal signal correction partial least squares analysis (OPLS),and potential biomarkers would be found according to the results of the score and load charts.RESULTS The result of metabolomics analysis showed that the urine of rats was clustering since the third week after model was constructed.Through analysis and identification of important variances,13 potential biomarkers ware screened out.CONCLUSION Distribution of endogenous metabolites of rats with renal fibrosis tends to be the normal level after intervention of tannins from pomegranate bark.It is feasible and effective to find potential biomarkers by metabolomics method.
renal fibrosistannins from pomegranate barkmetabolomics
NETL
NSTL
万方数据
维普
