Simultaneous determination of contents of isofraxidin, rosmarinic acid and naringin in Laomanqing syrup by using HPLC wavelength switching method
OBJECTIVE To establish an HPLC wavelength switching method to simultaneously determine contents for isofraxidin,rosmarinic acid and naringin in Laomanqing syrups.METHODS Waters SunFireTMC1s(4.6 mm× 150 mm,5 μm)column was used.Mobile phase was acetonitrile-water containing 0.1％phosphoric acid(18 ∶ 82)at a flow rate of 1.0 mL·min-1.Detection wavelength was 342 nm(0-12 min)for isofraxidin,284 nm(12-18 min)for naringin and 342 nm(18-35 min)for rosmarinic acid.RESULTS Isofraxidin had a good linearity in the range of 0.019-0.335 μg,the average recovery was 99.57％with RSD of 1.5％(n =9).Rosmarinic acid had a good linearity in the range of 0.022-0.397 μg,the average recovery was 99.55％with RSD of 1.3％(n =9).Naringin had a good linearity in the range of 0.085-1.531 μg,and the average recovery was 99.19％with RSD of 1.5％(n =9).CONCLUSION The method is simple,accurate and reproducible.It can be used to determine contents of isofraxidin,rosmarinic acid and naringin simultaneously in Laomanqing syrups under the same chromatogram conditions.
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万方数据
维普
