Effects of Banxia Xiexin decoction on small intestinal glucose absorption and insulin signal pathway in pre-diabetic rats induced by 3-deoxyglucosone
OBJECTIVE To investigate the effects of Banxia Xiexin Decoction on intestinal glucose absorption and insulin signal pathway in prediabetic rats induced by 3-deoxyglucosone(3-DG).METHODS SD rats were routinely fed for 3-5 days,and then 3DG(50 mg·kg-1)was administered continuously for two weeks to establish prediabetic rat model.The successful mod-eling rats were randomly divided into model group(n=6)and experimental group(n=6)according to their body weight,and another 6 normal rats were selected as blank group.The blank group and the model group were given 0.9％NaCl by intragastric administration at the dosage of 10 mL·kg-1·d-1,while the experimental group was accompanied by Banxia Xiexin Decoction at 5 g·kg-1·d-1 for 14 days.After 14 days of intervention,oral glucose tolerance test(oral glucose tolerance test,OGTT),glucose absorption test and Western blot test(to detect the expression of GLUT2 protein in rat duodenum and insulin receptor signal path-way p-IR,p110,p85,p-AKT)were performed.RESULTS (1)Compared with that of the blank group,the blood glucose level of the 3DG group was significantly higher(P＜0.05),and that of the Banxia Xiexin Decoction group was significantly lower than that of the 3DG group(P＜0.05).(2)Compared with that of the blank group,the content of 3-OMG in serum of the 3DG group was significantly increased(P＜0.05),and that of Banxia Xiexin Decoction group was significantly lower than that of the 3DG group(P＜0.05).(3)Compared with that of the blank group,the expression of GLUT2 in duodenal membrane of the 3DG group was significantly increased(P＜0.01),and that of the Banxia Xiexin Decoction group was significantly lower than that of the 3DG group(P＜0.01).(4)Compared with that of the blank group,the protein expression of p-IR,p110,p85 and p-AKT in the 3DG group was significantly decreased(P＜0.01),and that in Banxia Xiexin Decoction group was significantly higher than that in the 3DG group(P＜0.01).CONCLUSION 3DG can accelerate glucose absorption by damaging insulin signal pathway,which is one of the mechanisms in impaired glucose regulation.Banxia Xiexin Decoction can improve the acceleration of glucose absorption mediated by GLUT2 by regulating insulin signal pathway,which may be one of the mechanisms in improving 3DG-induced impaired glucose regulation.
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NSTL
万方数据
维普
