Immunotherapeutic effect of dendritic cell vaccine assisted by Glycyrrhiza inflata polysaccharides in H22 hepatoma-bearing mice
OBJECTIVE To prepare dendritic cell(DC)vaccine assisted by Glycyrrhiza inflata polysaccharide and to study its immunotherapeutic effect in H22 hepatoma-bearing mice.METHODS Bone marrow-derived dendritic cells(BMDC)were isolated from mouse bone marrow and cultured,DC was sensitized with H22 hepatoma antigen,and then a certain concentration of Glycyrrhiza inflata polysaccharides adjuvant was added to prepare DC vaccine.The morphological changes of DC were observed under an inverted microscope,and the phenotype of DC vaccine was detected by flow cytometry.H22 hepatoma-bearing mice was established and randomly divided into model group,positive group,DC group,TNF-α group,GiP group and GiP-B1 group by digital table method.The tumor-bearing mice were immunized with DC vaccine assisted by Glycyrrhiza inflata polysac-charides.The mice were sacrificed on the fifth day after the last administration,and the organ index and tumor inhibition rate were detected.The contents of IL-12(P70),IFN-γ,IL-4 and IL-10 cytokines in serum were detected by enzyme linked immunosor-bent assay(ELISA).The pathomorphological changes of liver and tumor tissue were observed by HE staining,and the expres-sion levels of Bax and Bcl-2 protein in tumor tissue were detected by immune-histochemistry.RESULTS Glycyrrhiza inflata polysaccharides as an adjuvant of DC vaccine could up-regulate the expression of DC surface markers(P＜0.05).The DC vaccine assisted by Glycyrrhiza inflata polysaccharides could slow down the tumor growth rate in tumor-bearing mice,increase the spleen index and thymus index of tumor-bearing mice(P＜0.05),increase the contents of IL-12 and IFN-γ in serum of tumor-bearing mice(P＜0.05),decrease the contents of IL-10 and IL-4(P＜0.05),and increase the ratio of Bax/Bcl-2 in tumor tissue(P＜0.01).CONCLUSION Glycyrrhiza inflata polysaccharide,as an adjuvant of DC vaccine,can induce DC maturation and activa-tion in vitro,and has a certain immunotherapeutic effect in tumor-bearing mice.Its anti-tumor mechanism may be related to up-regulating the secretion of IL-12 and IFN-γ,mediating cellular immunity and promoting tumor cell apoptosis.
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