Study on the immune-enhancing effect and mechanism of Rabdosia rubescens compound based on UPLC-Q-TOF-MS and network pharmacology
OBJECTIVE To study the chemical constituents of Rabdosia rubescens compound and its mechanism in enhanc-ing immune function.METHODS Chicken erythrocyte phagocytosis,lymphocyte transformation and splenocyte antibody pro-duction were used to study the immune function enhancement of Rabdosia rubescens compound in mice.The chemical constituents of Rabdosia rubescens compound were analyzed and identified by UPLC-Q-TOF-MS.The chemical composition targets were obtained by TCMSP,SEA and SwissTargetPrediction databases,the immune related targets were collected by GeneCards,OMIM and TTD databases,the intersection targets of drug and disease were obtained by Venn online website,and the core target network map was constructed by the combination of String database and Cytoscape software.The core targets were analyzed by GO and KEGG using David database,the"active component-target-pathway"network diagram was drawn by Cytoscape soft-ware,and the molecular docking verification of core targets and key components was carried out by Autodockvina software.RESULTS Compared with the blank group,the middle dose group of Rabdosia rubescens compound could significantly increase the phagocytic percentage and phagocytic index of peritoneal macrophages(P＜0.01),the proliferation of spleen lymphocytes(P＜0.01)and the number of hemolytic plaques(P＜0.01).The results showed that Rabdosia rubescens compound could enhance the non-specific and specific immune function of mice.A total of 120 chemical constituents were identified from the compound.The network pharmacology showed that the key components of the drug were 6-aldehyde isomalidinone A and methyl isoalizarin,etc.,and the core targets included AKT1,VEGFA and IL1B,etc.,which were mainly involved in PI3K-Akt,HIF-1,IL-17 and other signal pathways.The results of molecular docking showed that the core targets had good binding activity with the key components.CONCLUSION Rabdosia rubescens compound may enhance immune function in a multi-component,multi-target and multi-pathway manner.
NETL
NSTL
万方数据
维普
