Metabolomic study on liver of rats in physiological state treated with Euphorbia kansui before and after processing
OBJECTIVE To explore the attenuation mechanism of vinegar through the metabolomic study on liver of rats in physiological state treated with Euphorbia kansui before and after processing.METHODS Twenty-four SPF SD rats were ran-domly divided into blank group,E.kansui group and vinegar stir-fried E.kansui group with 8 rats in each group.The blank group was given 0.5％carboxymethylcellulose sodium(CMC-Na)by gavage,and the E.kansui group and vinegar stir-fried E.kansui group were given the corresponding test solution for 20 days.The livers were collected on the 20th day.Rat liver samples were analyzed by ultra-high performance liquid chromatography-quadrupole-orbital trap high resolution mass spectrometry(UPLC-Q-Exactive-MS).The data were processed by Compound Discoverer 3.0 software,and HMDB and other databases were searched.The differential metabolites were analyzed by principal component analysis(PCA),orthogonal partial least squares-discriminant analysis(OPLS-DA),and MetaboAnalyst to analyze the significant enrichment pathways of the obtained differential metabolites.RESULTS In the end,28 differential metabolites with biological significance were found between the E.kansui group and the blank group,18 metabolites were found with significant differences between the vinegar stir-fried E.kansui group and the blank group,and there were 13 metabolites in the intersection.CONCLUSION By increasing the levels of taurodeoxycholic acid and stearylcarnitine in liver,fermented vinegar can reduce the potential risk of early brain injury and type 2 diabetes.It can promote the regulation of immune function by increasing the level of glycocholic acid in liver.It can promote neurovascular remodeling and functional recovery after ischemic stroke by increasing the level of 20-hydroxyeicosaptaenoic acid in liver.By increasing the levels of 3-hydroxybutyric acid and oleo ethanolamine in liver,it shows anti-tumor,anti-oxidation and neuroprotection effects.The metabolic pathways involved in its attenuated mechanism are arachidonic acid metabolism,primary bile acid biosynthesis,ketone body synthesis and degradation,taurine and hypotaurine metabolism.
Euphorbia kansuiprocessing and detoxificationliver metabolomicstarget biomarkersmetabolic pathways
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