Study on the spectrum-effect relationship of anti-inflammatory and analgesic effects of the leaves of Litchi chinensis Sonn.from different places of origin
OBJECTIVE The fingerprint of the leaves of Litchi chinensis Sonn.was established by high performance liquid chromatography(HPLC),and the spectrum-effect relationship between fingerprint and anti-inflammatory and analgesic effects was explored.METHODS HPLC fingerprint of the leaves of Litchi chinensis Sonn.from different places of origin were estab-lished.The acute inflammation model of auricle swelling induced by xylene and the analgesic model of writhing response induced by acetic acid in mice were established to assess the anti-inflammatory and analgesic activities of the leaves of Litchi chinensis Sonn.Bivariate correlation analysis,grey relational analysis and partial least squares regression analysis were applied to explore the spectrum-effect relationship between common peaks in fingerprint and the pharmacodynamic indexes of anti-inflammatory and analgesic effects.RESULTS The fingerprint of the leaves of Litchi chinensis Sonn.was established,18 common peaks were identified,7 components were recognized,namely Gallic acid(peak 2),protocatechuic acid(peak 3),catechin(peak 4),epicat-echin(peak 8),rutin(peak 12),quercetin(peak 16)and kaempferol(peak 18).Bivariate correlation analysis revealed that peak 17 was significantly correlated with anti-inflammatory effect,while peaks 1,4,6,8,14,15,16 showed a low degree of correla-tion with anti-inflammatory effect.Peaks 1 and 17 exhibited significant correlation with analgesia,while peaks 4,7,8,9,13,14,15,16 and 18 showed a low degree of relation with analgesia.The grey relational analysis indicated that the correlation between the common peaks and anti-inflammatory and analgesic effects was more than 0.8 except that the correlation degree of peaks 2 and 3 was more than 0.6.CONCLUSION The HPLC fingerprint of the leaves of Litchi chinensis Sonn.was estab-lished.Combined with three spectral efficiency analysis methods,it was shown that the chemical constituents represented by peaks 1,4(catechin),6,8(epicatechin),16(quercetin)and 17 may be the anti-inflammatory or analgesic substances of the leaves of Litchi chinensis Sonn.This result could provide a basis for effective medical material screening and quality control for the leaves of Litchi chinensis Sonn.
The leaves of Litchi chinensis Sonn.spectrum-activityanalgesiaanti-inflammation
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