摘要
目的:采用高效液相色谱法(HPLC)建立荔枝叶药材的指纹图谱,并探究指纹图谱与抗炎、镇痛作用的谱效关系.方法:建立不同产地荔枝叶药材的HPLC指纹图谱;构建二甲苯致小鼠耳廓肿胀急性炎症模型及醋酸致小鼠扭体镇痛模型,考察荔枝叶抗炎、镇痛活性;采用双变量相关分析、灰色关联度分析与偏最小二乘回归分析的方法探究指纹图谱共有峰与抗炎、镇痛药效指标的谱效关系.结果:建立了荔枝叶药材的指纹图谱,确定了 18个共有峰,并指认出7个成分,分别为没食子酸(2号峰)、原儿茶酸(3号峰)、儿茶素(4号峰)、表儿茶素(8号峰)、芦丁(12号峰)、槲皮素(16号峰)、山柰酚(18号峰).经双变量相关分析发现,17号峰与抗炎作用呈显著相关,1,4,6,8,14,15,16号峰与抗炎作用呈现低度相关;1,17号峰与镇痛作用呈显著相关,4,7,8,9,13,14,15,16,18号峰与镇痛作用呈低度相关.经灰色关联度分析发现,除2,3号峰关联度大于0.6,其余共有峰与抗炎、镇痛作用的关联度均大于0.8.经PLS分析发现,1,4,6,8,16,17号峰对抗炎、镇痛作用的贡献较大.结论:建立了荔枝叶HPLC指纹图谱,结合3种谱效分析方法表明1,4(儿茶素)、6,8(表儿茶素)、16(槲皮素)、17号峰所代表的化学成分可能是荔枝叶发挥抗炎或镇痛作用的药效物质,可为荔枝叶药效物质基础筛选和质量控制研究提供依据.
Abstract
OBJECTIVE The fingerprint of the leaves of Litchi chinensis Sonn.was established by high performance liquid chromatography(HPLC),and the spectrum-effect relationship between fingerprint and anti-inflammatory and analgesic effects was explored.METHODS HPLC fingerprint of the leaves of Litchi chinensis Sonn.from different places of origin were estab-lished.The acute inflammation model of auricle swelling induced by xylene and the analgesic model of writhing response induced by acetic acid in mice were established to assess the anti-inflammatory and analgesic activities of the leaves of Litchi chinensis Sonn.Bivariate correlation analysis,grey relational analysis and partial least squares regression analysis were applied to explore the spectrum-effect relationship between common peaks in fingerprint and the pharmacodynamic indexes of anti-inflammatory and analgesic effects.RESULTS The fingerprint of the leaves of Litchi chinensis Sonn.was established,18 common peaks were identified,7 components were recognized,namely Gallic acid(peak 2),protocatechuic acid(peak 3),catechin(peak 4),epicat-echin(peak 8),rutin(peak 12),quercetin(peak 16)and kaempferol(peak 18).Bivariate correlation analysis revealed that peak 17 was significantly correlated with anti-inflammatory effect,while peaks 1,4,6,8,14,15,16 showed a low degree of correla-tion with anti-inflammatory effect.Peaks 1 and 17 exhibited significant correlation with analgesia,while peaks 4,7,8,9,13,14,15,16 and 18 showed a low degree of relation with analgesia.The grey relational analysis indicated that the correlation between the common peaks and anti-inflammatory and analgesic effects was more than 0.8 except that the correlation degree of peaks 2 and 3 was more than 0.6.CONCLUSION The HPLC fingerprint of the leaves of Litchi chinensis Sonn.was estab-lished.Combined with three spectral efficiency analysis methods,it was shown that the chemical constituents represented by peaks 1,4(catechin),6,8(epicatechin),16(quercetin)and 17 may be the anti-inflammatory or analgesic substances of the leaves of Litchi chinensis Sonn.This result could provide a basis for effective medical material screening and quality control for the leaves of Litchi chinensis Sonn.
基金项目
国家中医药管理局高水平中医药重点学科建设项目(zyyzdxk-2023165)
广西壮瑶药重点实验室(桂科基字[2014]32号)
广西中医药多学科交叉创新团队项目(GZKJ2309)
广西高等学校千名中青年骨干教师培育计划(桂教人[2019]5号)
壮瑶药协同创新中心(桂教科研[2013]20号)
广西壮族自治区民族药资源与应用工程研究中心(桂发改高技函[2020]2605号)
广西中医药重点学科(GZXK-Z-20-64)
广西一流学科(桂教科研[2018]12号)
NETL
NSTL
万方数据