Preparation of smart near-infrared light-responsive composite hydrogels based on black phosphorus nanosheets and evaluation of in vitro anti-osteosarcoma activity
OBJECTIVE To prepare smart near-infrared light(NIR)-responsive composite hydrogels based upon black phosphorus nanosheets(BPNSs)for synergistic anti-osteosarcoma photothermal-chemotherapy.METHODS BPNSs were pre-pared by liquid phase exfoliation and modified with polyethylene glycol diamine[PEG-(NH2)2]as a modifier under the principle of electrostatic interaction.And modified BPNSs were loaded efficiently with adriamycin(DOX)by electrostatic interaction.BPNSs modified by PEG and loaded with DOX were co-blended in sodium alginate/poroxam composite hydrogel matrix for pre-paring smart NIR light responsive composite hydrogels based upon BPNSs by cold method.And in vitro anti-osteosarcoma potency was evaluated by a murine model of osteosarcoma osteoblast(K7M2-WT).RESULTS PEG surface modification of BPNSs improved biostability and slowed down oxidative degradation;drug loading and encapsulation rates of BPNSs were(101.6±3.2)％and(25.3±0.9)％respectively;smart NIR photoresponsive composite hydrogel based upon BPNSs could rap-idly complete sol-gel phase transition at 37 ℃ to form a semi-solid drug reservoir with decent through-needle property,excellent photothermal conversion,pH/NIR smart response drug release performance,excellent in vitro biocompatibility and in vitro syner gistic anti-osteosarcoma photothermal-chemotherapy.CONCLUSION The preparation process of intelligent NIR photorespon-sive composite hydrogels based upon BPNSs is both simple and controllable.It may achieve intelligent photocontrol and sustained drug release through NIR induction,making synergistic in situ anti-osteosarcoma photothermal-chemotherapy more efficient and promising.
black phosphorus nanosheetshydrogelsynergistic photothermal-chemotherapyosteosarcoma
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