Research advances of polyethylenimine-based nanosystems for gene delivery
Gene therapy has demonstrated great therapeutic potentials for such major refractory diseases as malignant tumors,infectious,autoimmune and rare disease,etc.Gene delivery vectors are essential for a successful implementation of gene therapy.Polythylenimine(PEI)is a widely studied cationic gene delivery vector,showing stable and efficient gene transfection in numer-ous cell lines and transfection conditions.PEI25k is regarded as a"gold standard"for gene transfection.Although PEI has broad application prospects in the field of gene delivery,there are still urgent problems to be tackled,including low transfection efficiency in vivo,high cytotoxicity,low targeting capability and lysosomal degradation of loaded gene.This review summarized the latest advances of designing novel polyethylenimine-based nanosystems for gene therapy,including high molecular weight linear PEI,PEI modified with polysaccharides,hydrophilic polymers or dextran,crosslinked low molecular weight branched PEI,PEI-based inorganic nanoparticles and co-delivery vectors of drug/gene based upon PEI.It was intended to provide theoretical rationales for further constructing gene delivery carriers with high efficiency and low toxicity.
polythyleniminegene therapycationic polymernonviral vectorgene delivery system
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