Study on in vitro transdermal consistency of minoxidil solution
OBJECTIVE To compare the consistency of transdermal effect of 5％minoxidil solution test product versus refer-ence product.METHODS Bama miniature pig skin was utilized as a material for transdermal penetration and vertical modified Franz diffusion cell for determining the penetration of minoxidil solution.Liquid chromatography/tandem mass spectrometry(LC-MS/MS)was employed for determining the relationship between total amount of drug permeation in receiving solution and reten-tion of drug in skin with time.RESULTS Test and reference products in skin for 24 h cumulative permeation were(49 721± 29 707)and(45 372±23 755)ng·cm-2;Average permeation rates were(2 352±1 309)and(2 182±1 080)ng·cm-2·h-1;24 h skin retention(218 276±137 675)and(188 040±49 523)ng·g-1;Average permeation rates of test and reference products at a cumulative permeation of about half total permeation(12 h)were(2 172±1 330)and(1 947±1 037)ng·cm-2·h-1.CONCLUSION Degree and rate of transdermal penetration of 5％minoxidil solution test/reference formulation fulfill the consis-tency requirements for topical formulations.And drug retention characteristics of minoxidil solution are similar in Bama miniature pig skin.
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