Interventional effects of Qingdu Shenjin Granules on adjuvant-induced arthritis model rat
OBJECTIVE To explore the therapeutic effects of Qingdu Shenjin Granules(QSG)on adjuvant arthritis(AA)rats.METHODS Male Sprague-Dawley(SD)rats were utilized for AA rat modeling with Freund's complete adjuvant.Then the animals were randomized into five groups of model,Wantong Jingu Tablet(0.15 g·kg-1)and QSG low/medium/high-dose(3.9,7.8,15.6 g·kg-1)(n=10 each).Another 10 normal SD rats were designated as normal control group.Each group a daily gavage for 30 consecutive days.Pain threshold,foot swelling degree and arthritis score were measured regularly.After dosing,serum immunoglobulin G(IgG),immunoglobulin M(IgM),tumor necrosis factor alpha(TNF-α)and interleukin-1 beta(IL-1β)were detected by enzyme-linked immunosorbent assay(ELISA).Joint pathological injury was examined after hematoxylin-eosin stain of joint sections.And histopathological changes of ankle joint were scored.RESULTS Compared with normal con-trol group,pain threshold of AA rats declined markedly.Foot swelling degree,arthritis index and serum levels of inflammatory cytokines spiked markedly.Synovial layer,cartilage and bone layer of ankle joint became severely injured and there was a marked rise of ankle joint pathological score(P＜0.05,P＜0.01).As compared with model group,QSG could significantly elevate the pain threshold of AA rats,improve foot swelling,reduce arthritis index,lower serum levels of inflammatory cytokines,lessen the injury of synovial layer,cartilage and bone layer of ankle joint and lower ankle joint pathological tissue score(P＜0.05,P＜0.01).CONCLUSION QSG has therapeutic effect on AA rats.And its mechanism may be correlated with lowering the produc-tion or secretion of inflammatory cytokines.
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