Property-efficacy relationship of antirheumatic traditional chinese medicine Clematidis Radix et Rhizoma and Siegesbeckiae Herba on arthralgia syndrome rats
OBJECTIVE To compare the differences in the effects of Clematidis Radix et Rhizoma and Siegesbeckiae Herba on rheumatoid arthritis(RA)model rats with wind-cold-dampness/wind-dampness-heat arthralgia and examine the correlation with the nature of traditional Chinese medicine-disease-syndrome-efficacy.METHODS A total of 112 Sprague-Dawley(SD)rats were randomized into 14 groups of blank,type Ⅱ collagen(CⅡ),wind-cold-dampness model(FHS-M),wind-cold-dampness Chinese medicine intervention(including FHS-L,FHS-WG,FHS-WD,FHS-XG & FHS-XD),wind-dampness-heat model(FSR-M),wind-dampness-heat Chinese medicine intervention(including FSR-L,FSR-WG,FSR-WD,FSR-XG& FSR-XD)(n=8 each).In addition to blank group,the remainders were induced by natural bovine type Ⅱ collagen and a stimu-lation of wind-cold-dampness and wind-dampness-heat environmental factors.General profiles,joint swelling degree and arthritis score of rats in each group were recorded.The pathological changes of ankle joint tissue were observed by hematoxylin-eosin(HE)stain.The serum contents of TNF-α,ICAM-1 and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA).The expressions of VEGF-A,MMP-3 and TIMP-1 protein in ankle joint were quantified by immunohistochemistry(IHC).The expression of VEGF-A,MMP-3 and TIMP-1 mRNA in ankle joint was detected by real-time polymerase chain reaction(RT-PCR).RESULTS As compared with blank group,paw swelling degree and AI score of rats spiked sharply in C Ⅱ,FHS-M and FSR-M groups.Structural destruction of ankle joint was evident.Serum contents of TNF-α and ICAM-1 rose,mRNA/protein expressions of VEGF-A and MMP-3 spiked and mRNA/protein expression of TIMP-1 dropped.As compared with FHS-M/FSR-M group,paw swelling degree and AI score of rats dropped obviously in each dosing group.Pathological structure of ankle joint tissue diminished,serum contents of TNF-α and ICAM-1 declined,mRNA/protein expressions of VEGF-A and MMP-3 dropped and mRNA/protein expression of TIMP-1 rose.As compared with FHS-M group,FHS-XG group had the greatest improvement.As compared with FSR-M group,FSR-XG group had the greatest improvement.CONCLUSION Both Clemati-dis Radix et Rhizoma and Siegesbeckiae Herba may improve the status of RA rats.However,the therapeutic effect of Clematidis Radix et Rhizoma is superior to that of Siegesbeckiae Herba.And the therapeutic effect of Siegesbeckiae Herba with wind-dampness-heat arthralgia is better than that of Clematidis Radix et Rhizoma in RA rats.
Clematidis Radix et RhizomaSiegesbeckiae Herbawind-cold-dampness arthralgiawind-dampness-heat arthralgiaproperty-efficacy relationship
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