Immunomodulatory effect of Baijiezi Tufang Decoction on treating asthma
OBJECTIVE To explore the immunomodulatory effect of Baijiezi Tufang Decoction(BTD)on treating asthma.METHODS SD rat asthma model was constructed.And the animals were randomized into five groups of control,model,BTD applied to non-acupoint,BTD applied to Feishu acupoint and positive control(aminophylline applied to Feishu acupoint).Serum samples,lung,spleen,thymus,liver and kidney tissues were harvested after 14-day dosing.Lymphocyte proliferation and macro-phage activation were evaluated,serum levels of cytokines quantified,organ indices,morphology and skin DC cell count exam-ined and expression changes of CD90 and H2-Ab1 detected.RESULTS As compared with model group,acupoint application of BTD and aminophylline could induce T lymphocyte proliferation(P＜0.01),leading to the elevations of IgA,IgG,IgM,IFN-γand IL-2(P＜0.01)and yet the reductions of IL-4 and MBP(P＜0.01).In addition,lymphocyte infiltration,cell swelling num-ber,mucus secretion in bronchial lumen,airway edema and occlusion,pulmonary interstitial inflammatory infiltration and conges-tion improved in skin tissue of Feishu acupoint and aminophylline groups.Further study indicated that CD1a+CD83-immature DC cells dropped while CD1a+CD83+mature DC cells spiked and the expressions of CD90 and H2-Ab1 became markedly up-regulated in skin tissue.CONCLUSION Acupoint application of BTD may promote the deformation and migration of myeloid dendritic cells in skin tissue,enhance antigen presentation,activate immune function of body,exhibit low toxic/side effects and play some regulatory role in an imbalance of Th1/Th2.
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