Correlation between chemical constituents and effects of soothing liver and strengthening spleen after com-patibility of Atractylodes macrocephalae Koidz and Paeonia lactiflora Pall
OBJECTIVE To clarify the impact of multi-component contents and pharmacodynamics of soothing liver and invigorating spleen after compatibility of Atractylodes macrocephalae Koidz and Paeonia lactiflora Pall and examine the correla-tion between chemical components and pharmacodynamics.METHODS A rat model of liver depression and splenic deficiency was established.The levels of 5-hydroxytryptamine(5-HT),somatostatin(SS)and motilin(MTL)were detected by enzyme-linked immunosorbent assay(ELISA).High-performance liquid chromatography-diode array detection(HPLC-DAD)was uti-lized for determining the contents of 7 active components.The correlations between chemical composition content and pharmaco-logical effects were examined by bivariate and grey correlations.RESULTS As compared with model group,the plasma concen-trations of 5-HT and SS declined obviously in all treatment groups(P＜0.05,P＜0.01)and more dramatically in compatibility group(P＜0.05).The concentration of MTL rose obviously only in compatibility group(P＜0.01).After compatibility,the contents of oxypaeoniflorin,paeoniflorin,benzoyl paeoniflorin and atractylenolide Ⅰ spiked markedly(P＜0.05,P＜0.01).Che-mometry revealed a strong correlation between benzoyl paeoniflorin,atractylenolide Ⅰ and 5-HT,SS,MTL with correlation degrees＞0.70.CONCLUSION The compatibility of Atractylodes macrocephalae Koidz and Paeonia lactiflora Pall plays a vital role of soothing liver and invigorating spleen through multi-components.Higher contents of active ingredient may be a key factor of enhancing drug efficacy.It provides references for determining the quality control components based upon drug efficacy.
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