白术-白芍配伍对其化学成分及疏肝健脾作用的相关性研究
Correlation between chemical constituents and effects of soothing liver and strengthening spleen after com-patibility of Atractylodes macrocephalae Koidz and Paeonia lactiflora Pall
朱瑶瑶 1潘娉娉 1吴恩慧 1章建华 2尹华1
作者信息
- 1. 浙江中医药大学药学院,浙江杭州 311400
- 2. 浙江中医药大学附属第一医院骨伤科,浙江杭州 310000
- 折叠
摘要
目的:明确白术-白芍配伍对多成分含量及疏肝健脾作用的影响,进而研究两者的相关性.方法:建立肝郁脾虚大鼠模型,ELISA法检测大鼠血浆中5-羟色胺(5-HT)、胃动素(MTL)、生长抑素(SS)水平;采用HPLC-DAD法同时测定药对中7个活性成分的含量,以双变量相关、灰色关联度法对化学成分含量-药效进行相关性分析.结果:与模型组相比,各给药组大鼠血浆中5-HT、SS浓度水平显著降低(P＜0.05,P＜0.01),且配伍组降低水平更甚(P＜0.05),MTL浓度水平仅配伍组显著升高(P＜0.01).药对配伍后,氧化芍药苷、芍药苷、苯甲酰芍药苷、白术内酯Ⅰ的含量显著增加(P＜0.05,P＜0.01).化学计量学方法显示白术内酯Ⅰ、苯甲酰芍药苷与5-HT、SS、MTL呈较强相关性,关联度大于0.70.结论:白术-白芍药对通过多成分综合发挥疏肝健脾作用,药对配伍后活性成分溶出量增加可能是其药效增强的关键因素,可为其基于药效的质控指标成分的确定提供参考依据.
Abstract
OBJECTIVE To clarify the impact of multi-component contents and pharmacodynamics of soothing liver and invigorating spleen after compatibility of Atractylodes macrocephalae Koidz and Paeonia lactiflora Pall and examine the correla-tion between chemical components and pharmacodynamics.METHODS A rat model of liver depression and splenic deficiency was established.The levels of 5-hydroxytryptamine(5-HT),somatostatin(SS)and motilin(MTL)were detected by enzyme-linked immunosorbent assay(ELISA).High-performance liquid chromatography-diode array detection(HPLC-DAD)was uti-lized for determining the contents of 7 active components.The correlations between chemical composition content and pharmaco-logical effects were examined by bivariate and grey correlations.RESULTS As compared with model group,the plasma concen-trations of 5-HT and SS declined obviously in all treatment groups(P＜0.05,P＜0.01)and more dramatically in compatibility group(P＜0.05).The concentration of MTL rose obviously only in compatibility group(P＜0.01).After compatibility,the contents of oxypaeoniflorin,paeoniflorin,benzoyl paeoniflorin and atractylenolide Ⅰ spiked markedly(P＜0.05,P＜0.01).Che-mometry revealed a strong correlation between benzoyl paeoniflorin,atractylenolide Ⅰ and 5-HT,SS,MTL with correlation degrees＞0.70.CONCLUSION The compatibility of Atractylodes macrocephalae Koidz and Paeonia lactiflora Pall plays a vital role of soothing liver and invigorating spleen through multi-components.Higher contents of active ingredient may be a key factor of enhancing drug efficacy.It provides references for determining the quality control components based upon drug efficacy.
关键词
白术/白芍/配伍/化学成分/含量测定/疏肝健脾/相关性Key words
Atractylodes macrocephalae Koidz/Paeonia lactiflora Pall/compatibility/chemical constituents/content deter-mination/soothing liver and strengthening spleen/correlation analysis引用本文复制引用
基金项目
国家自然科学基金资助项目(81973868)
出版年
2024
NETL
NSTL
万方数据