Quality markers of Lixieling Tablets based upon network pharmacology and principal component analysis
OBJECTIVE To predict and evaluate the quality markers of Lixieling Tablets by network pharmacology and prin-cipal component analysis.METHODS UPLC-QE-Orbitrap-MS technology was utilized for identifying the chemical components of Lixieling Tablets.ADME prediction of chemical components was performed through SwissADME platform.The lead compo-nents were obtained by analyzing pharmacological effects of compounds,collectability of reference materials and the testability of components.PharmaMapper database was utilized for predicting the action targets of lead components.The databases of Gen-eCards,OMIM CTD and DisGenet were employed for screening disease targets.Venn diagram was constructed for harvesting intersection targets as potential therapeutic targets of Lixieling Tablets.Metascape platform was utilized for conducting enrichment analysis by introducing potential targets and obtaining the potential intervention pathway of Lixieling Tablets.Cytoscape software was utilized for constructing a network of"component-target-pathway"to identify core targets and major active ingredients.Princi-pal component analysis was applied for evaluating the equivalence of the active ingredients and Lixieling Tablets in normal animals.RESULTS The network pharmacology predicted the ten active ingredients of Lixieling Tablets,including andrographolide,dehydroandrographolide,(+)-catechin,chlorogenic acid,oxysophocarpine,oxymatrine,sophocarpine,matrine,sophoridine and gallic acid.Principal component analysis revealed that active ingredients and Lixieling Tablets exhibited similar metabolic regu-latory effects in normal animals.CONCLUSION Predicting and evaluating the quality markers of Lixieling Tablets are feasible through network pharmacology and principal component analysis.The complex of active ingredients is equivalent to original prepa-ration to a certain extent.
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