High performance liquid chromatographic fingerprinting of Tangshen Dihuang Decoction and measure-ments of its multiple components
OBJECTIVE To establish the high performance liquid chromatography(HPLC)fingerprint and formulate a multi-indicator protocol for measuring the contents of Tangshen Dihuang Decoction(TDD)to provide references for its quality control(QC)and evaluations.METHODS Separation was performed on Symmetry® C18(250 mm×4.6 mm,5 μm)at 30 ℃ and eluted with acetonitrile-0.1％phosphoric acid at a flow rate of 1.0 mL·min-1,and a detection wavelength of 240 nm.Simi-larity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine(Edition 2012)was utilized for evalu-ating the similarity of HPLC fingerprints of 10 batches of samples and determining their specific contents.RESULTS HPLC fin-gerprint of 10 batches of TDD was performed.All similarities were greater than 0.99.A total of 20 common peaks were identi-fied.Peaks 1/6/7/10/14/17/18/19(paeonol)were attributed to peony bark,peaks 2/3/4/5(morroniside),peak 8(loganin),peaks 9/13 Comi Fructus,peak 11(calycosin-7-glucoside)Astragalus membranaceus,peaks 15/16(salvianolic acid B)and peak 20(tanshinone Ⅱ A)Salvia miltiorrhiza.Peak 12 was composed of Cornus officinalis and peony bark.QC standards were estab-lished by morroniside,loganin,calycosin-7-glucoside,paeonol,salvianolic acid Ⅱ A and salvianolic acid B.Mass fractions of morroniside,loganin,calycosin-7-glucoside,paeonol,salvianolic acid Ⅱ A and salvianolic acid B in each batch were(69.18-70.21),(81.82-82.84),(4.31-4.32),(346.33-347.26),(189.37-190.58)and(8.39-8.47)μg·mL1 respectively.CONCLUSION Fingerprinting and content determination of TDD are simple,sensitive and reproducible.It may be employed as rationales for follow-up QC of TDD.
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