六神曲发酵过程中酶学性质与微生物群落变化相关性研究

扫码查看

原文链接

NETL
NSTL
万方数据

中文摘要：目的:以六神曲为研究对象，对发酵过程中酶学性质与微生物群落变化之间的相关性进行研究。方法:采用酶活检测方法对六神曲酶学特性进行评价，采用MiSeq高通量测序技术对微生物类群进行解析，利用相关性分析发酵过程中酶的性质变化与微生物群落之间的相关性。结果:发酵初期，微生物大量繁殖，淀粉酶、糖化酶、蛋白酶、脂肪酶、滤纸酶和纤维素酶等活性指标急剧上升;发酵中末期，微生物种类下降，曲霉菌占据主导地位。糖化酶活性在36 h达到最高值53。818μmol·g-1，淀粉酶在30h达到最高值87。841 µmol·g-1，脂肪酶、滤纸酶和纤维素酶在24h分别达到最高值0。048 μmol·g-1、1。623μmol·g-1和1。521 µmol·g-1，均呈现先升后降的变化趋势;经过42h，蛋白酶开始呈现出快速升高后趋于稳定。相关性分析表明，Saccha-romycopsis与糖化酶、Aspergillus与蛋白酶、Wickerhamomyces与纤维素酶、滤纸酶等指标之间呈显著正相关性，Alternaria与糖化酶指标之间呈显著负相关性。优势菌株Saccharomycopsis、Aspergillus、Wickerhamomyces是影响六神曲发酵过程中酶学性质的主要因素。结论:该研究为六神曲微生物群落及多酶体系代谢研究提供了数据参考，为改良六神曲发酵工艺及指导规范化生产提供了理论依据。

外文标题：Correlation between changes in enzymatic properties and microbial communities during fermentation of Massa Medicata Fermentata

外文摘要：OBJECTIVE To explore the correlation between enzymatic properties and microbial colonial changes during fer-mentation with Massa Medicata Fermentata as a research object.METHODS The enzymatic characteristics of Massa Medicata Fermentata were evaluated by enzymatic activity detection.MiSeq high-throughput sequencing was utilized for examining micro-bial groups.And correlation analysis was performed for analyzing the correlation between the changes of enzyme properties and microbial colonies during fermentation.RESULTS During an initial stage of fermentation,microorganisms proliferated and such activity indices as amylase,glucoamylase,protease,lipase,filter paper enzyme and cellulase spiked sharply.In middle/late stage of fermentation,microbial species diminished and Aspergillus dominated.The peak glucoamylase activity reached 53.818 μmol·g-1 at 36 h,amylase 87.841 μmol·g-1 at 30 h and lipase,filter paper enzyme and cellulase 0.048,1.623 and 1.521 μmol·g-1 at 24 h respectively,showing an initial rising trend and a subsequent decline.After 42 h,protease dropped sharply and then stabi-lized.Correlation analysis showed that significant positive correlations existed between Saccharomycopsis and glucoamylase,Aspergillus and protease,Wickerhamomyces and cellulase,filter paper enzyme and other indicators while a significant negative cor-relation existed between Altemaria and glucoamylase indicators.Dominant strains of Saccharomycopsis,Aspergillus and Wicker-hamomyces were the major factors affecting the enzymatic properties of Massa Medicata Fermentata.CONCLUSION This study provides data supports for exploring microbial colonies and multienzyme system metabolism of Massa Medicata Fermentata.And theoretical rationales are offered for improving the fermentation technology of Massa Medicata Fermentata and guiding stan-dardized production.

外文关键词：

Massa Medicata Fermentatafermentationenzymatic propertiesmicroorganismscorrelation analysis

作者：

丁海玲、时海燕、王爽、徐丹丹、赵霞、崔彦伟、徐男

展开 >

作者单位：

齐鲁理工学院医学院,山东济南 250299

山东第一医科大学第一附属医院(山东省千佛山医院)临床药学部,山东省儿童药物临床评价与研发工程技术研究中心,山东省医药卫生临床药学重点实验室,山东济南 250014

山东中医药大学药学院,山东济南 250355

山东省中医药研究院中药制剂研究所,山东济南 250014

山东百味堂中药饮片有限公司研发部,山东济南 250100

展开 >

关键词：

六神曲发酵过程酶学性质微生物相关分析

基金：

山东省科技型中小企业创新能力提升工程济南市科技型中小企业创新能力提升工程济南市"新高校20条"引进创新团队项目山东省重点研发计划重大科技创新工程山东省自然科学基金重点项目

项目编号：

2022TSGC10572022TSGC10572023330212022CXGC020514ZR2020KH017

出版年：

2024

DOI：

10.13286/j.1001-5213.2024.17.04

中国医院药学杂志

中国药学会

中国医院药学杂志

CSTPCD北大核心

影响因子：1.198

ISSN：1001-5213

年,卷(期)：2024.44(17)