Mechanism of Xiexin Tang effective components on arresting atherosclerosis based upon network pharmacology
OBJECTIVE To explore the mechanism of Xiexin Tang effective components(XXT ECs)acting on atheroscle-rosis(AS)through a combination of network pharmacology and cell experiments.METHODS The targets of XXT ECs were obtained from the database of TCMSP while the disease targets of AS from the databases of GeneCards,TTD and Drugbank data-bases.The common targets of XXT ECs and AS were identified by the Venn Diagram software.The effective components-target network was constructed with Cytoscape 3.7.2 software.A common target interaction protein-protein interaction(PPI)network was created by the database of STRING 11.0.GO function and KEGG pathway enrichment analyses were performed with Metascape.An in vitro model was established by inducing EA.hy926 cells with palmitic acid(PA)for validating the results of network pharmacology.RESULTS The screening process yielded 285 corres1onding protein targets,589 disease targets and 49 intersection targets.The core targets included LDLR,RPPARG,VEGFA,IL-1β,IL-6 and TNF-α.KEGG analysis primarily included pathways related to inflammation,lipid metabolism and endothelial protection.Enzyme-linked immunosorbent assay(ELISA)and quantitative polymerase chain reaction(qPCR)demonstrated that XXT ECs significantly suppressed inflammatory responses of palmitic acid-induced EA.hy926 endothelial cells leading to down-regulations of IL-1β,IL-6 and TNF-α.CONCLUSION XXT ECs may arrest atherosclerosis through targeting key inflammatory factors.The experiment provided valuable rationales for elucidating the anti-AS mechanism of XXT ECs.
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