Identification of chemical constituents in Yinxing Yangnao Prescription by UPLC-Q-TOF-MS
OBJECTIVE To rapidly identify the chemical constituents of Yinxing Yangnao Prescription and clarify the pos-sible cleavage pathway of some chemical constituents.METHODS Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was developed for rapid analysis and identification of chemical constituents of Yinxing Yangnao Prescription.The chromatographic column was ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7 μm).Mobile phase was composed of 0.1％formic acid water-acetonitrile with gradient elution with a flow rate of 0.4mL·min-1.Positive and negative ion modes were utilized for scanning with an range of m/z 50-1 500.According to the accurate mass-to-charge ratio and secondary fragment ion information,in conjunctions with reference substances and literature data,the chemical constituents in Yinxing Yangnao Prescription were identified.RESULTS A total of 86 chemical constituents were identified,including phenylpropanoids,flavonoids,terpenoids,alkaloids,glycosides,phthalides,phenols,oligosaccharide esters and ste-roids.The cleavage pathways of flavonoids,phenylpropanoids and terpenoids were analyzed.CONCLUSION The method is rapid and accurate so that it may be used as a rapid analysis and identification of chemical constituents of Yinxing Yangnao Prescrip-tion.Also the possible cleavage pathways of some chemical constituents are clarified.It provides theoretical rationales for quality controls and material basis researches of Yinxing Yangnao Prescription.
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