基于UPLC-Q-TOF-MS技术的银星养脑方化学成分分析
Identification of chemical constituents in Yinxing Yangnao Prescription by UPLC-Q-TOF-MS
陶叶琴 1熊丹 1王鑫 2严建业 2高明果 1蒋翠平 1谢宗明1
作者信息
- 1. 湖南中医药大学附属第二中西医结合医院制剂科,湖南浏阳 410300;浏阳市中医医院制剂科,湖南浏阳 410300
- 2. 湖南中医药大学科创中心,湖南长沙 410013
- 折叠
摘要
目的:对银星养脑方的化学成分进行快速分析与鉴定,阐明其所含部分化合物可能的裂解途径.方法:采用超高效液相色谱串联四极杆飞行时间质谱联用技术(UPLC-Q-TOF-MS)对银星养脑方的化学成分进行快速分析与鉴定,色谱柱为ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7 µm),以0.1％甲酸水-乙腈为流动相,梯度洗脱,体积流量0.4 mL·min-1.采用正、负离子模式扫描,扫描范围m/z 50～1 500.根据精确质荷比和二级碎片离子信息,结合对照品和文献数据鉴定银星养脑方中的化学成分.结果:共鉴定出86个化学成分,包括苯丙素类、黄酮类、萜类、生物碱类、糖苷类、苯酞类、酚类、寡糖酯类、甾体类等,并对黄酮类、苯丙素类和萜类等成分进行裂解途径分析.结论:该方法快速准确,可作为银星养脑方化学成分快速分析与鉴别,并阐明了部分化学成分可能的裂解途径,为银星养脑方质量控制和物质基础研究提供理论依据.
Abstract
OBJECTIVE To rapidly identify the chemical constituents of Yinxing Yangnao Prescription and clarify the pos-sible cleavage pathway of some chemical constituents.METHODS Ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)was developed for rapid analysis and identification of chemical constituents of Yinxing Yangnao Prescription.The chromatographic column was ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7 μm).Mobile phase was composed of 0.1％formic acid water-acetonitrile with gradient elution with a flow rate of 0.4mL·min-1.Positive and negative ion modes were utilized for scanning with an range of m/z 50-1 500.According to the accurate mass-to-charge ratio and secondary fragment ion information,in conjunctions with reference substances and literature data,the chemical constituents in Yinxing Yangnao Prescription were identified.RESULTS A total of 86 chemical constituents were identified,including phenylpropanoids,flavonoids,terpenoids,alkaloids,glycosides,phthalides,phenols,oligosaccharide esters and ste-roids.The cleavage pathways of flavonoids,phenylpropanoids and terpenoids were analyzed.CONCLUSION The method is rapid and accurate so that it may be used as a rapid analysis and identification of chemical constituents of Yinxing Yangnao Prescrip-tion.Also the possible cleavage pathways of some chemical constituents are clarified.It provides theoretical rationales for quality controls and material basis researches of Yinxing Yangnao Prescription.
关键词
超高效液相色谱串联四极杆飞行时间质谱联用技术(UPLC-Q-TOF-MS)/银星养脑方/化学成分Key words
UPLC-Q-TOF-MS/Yinxing Yangnao Prescription/chemical composition引用本文复制引用
基金项目
湖南省自然科学基金项目(2024JJ9573)
湖南省临床医疗技术创新引导项目(2021SK51501)
长沙市卫健委中医药科研项目(A202304)
出版年
2024
NETL
NSTL
万方数据