Screening for material basis of anti-inflammatory effect of Salvia plebeia based upon zebrafish inflamma-tion model
OBJECTIVE To screen for the anti-inflammatory substance of Salvia plebeia based upon zebrafish inflammation model.METHODS The chemical constituents of 95％,70％,50％,30％ethanol extraction parts and water extraction parts of Salvia plebeia were characterized by UPLC-Q-TOF-MS/MS for determining the common component Salvia plebeia.And the inflammatory model of zebrafish was established for evaluating the anti-inflammatory activity of each extract fraction according to the expression levels of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and interleukin-10(IL-10).The correlation between peak area of common components in characterization map and anti-inflammatory activity index was established by Pear-son's bivariate analysis and grey correlation degree and anti-inflammatory quality markers(Q-markers)of Salvia plebeia was veri-fied.RESULTS 95％ethanol extract had the best anti-inflammatory activity.A total of 22 common components were identified from 5 extract sites.Homoplantaginin,hispidulin,isorhamnetin,luteolin,apigenin,camosol and 6-hydroxyluteolin were identi-fied as potential anti-inflammatory substances of Salvia plebeia,among which the first 6 easily obtained components were selected for anti-inflammatory activity verification.And the results showed that they could significantly lower the expressions of TNF-αand IL-6 and promote the expression of IL-10 in Zebrafish inflammatory model(P＜0.01).CONCLUSION The elucidating of anti-inflammatory substances of Salvia plebeia lays of foundation for its quality evaluation and comprehensive development and uti-lization of resources.
Salvia plebeiachemical characterizationanti-inflammatory activitycorrelation analysis
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