Preparation and in vitro evaluation of a berberine-loaded single-walled carbon nanotubes drug delivery system
OBJECTIVE To prepare a drug delivery system of a single-walled carbon nanotubes loaded berberine(F127/SWCNTs-COOH/Ber)and explore the inhibitory effect on prostate cancer.METHODS Berberine was loaded onto F127/SWCNTs-COOH by ultrasonic method and formulation process was optimized by single-factor test using encapsulation efficiency and drug loading as parameters.Particle size,Zeta potential,in vitro dissolution and adsorption characteristics of F127/SWCNTs-COOH/Ber were explored.And cytotoxicity was evaluated in PC-3 cells.RESULTS The optimal formulation pro-cess for F127/SWCNTs-COOH/Ber was as follows:ratio of carrier to drug was 1∶1,ultrasonic power 200 W and ultrasonic fre-quency 30 times.Average particle size and Zeta potential were(182.3±11.64)nm and(-3.44±0.17)mV;encapsulation effi-ciency and drug loading 81.09％and 38.86％;cumulative dissolution rates within 30 min in simulated intestinal fluid and simu-lated gastric fluid 94.02％and 76.69％.The absorption of berberine in F127/SWCNTs-COOH followed the pseudo-second-order model.Anti-cancer activity of F127/SWCNTs-COOH/Ber was significantly higher than that of berberine alone,thereby enabling a more effective delivery of loaded berberine into tumor cells for therapeutic purposes.CONCLUSION F127/SWCNTs-COOH/Ber has successfully prepared,demonstrating its potential of enhancing the inhibitory effect on the proliferation of PC-3 cells in PC and cell uptake efficiency.This provides a promising delivery vector for treating prostate cancer.
berberineSWCNTs-COOHdrug delivery systemprostate cancer
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