Component analysis of different medicinal parts of Entada phaseoloides based upon UPLC-MS/MS tech-nology and evaluations of antipruritic and analgesic activities
OBJECTIVE To compare the variations of chemical constituents and pharmacodynamics of different parts from Entada phaseoloides(Linn.)Merr.,including seed kernels,seed coats and stems,and investigate the correlations between chemical constituents and pharmacodynamics.METHODS Chemical components and different parts of E.phaseoloides were examined through UPLC-MS/MS,principal analysis,partial least square discriminant analysis and other stoichiometrics.Hista-mine or chloroquine-evoked acute pruritus model in mice and complete Freund's adjuvant-induced inflammatory pain model in rats were employed for evaluating the antipruritic and analgesic activities of different parts of E.phaseoloides.Furthermore,the corre-lation between differential compounds and anti-inflammatory,antipruritic and analgesic activities was verified by Pearson's correla-tion analysis.RESULTS A total of 85 compounds were identified,including flavonoids,phenolic acids,triterpenes and entad-amides,etc.Among them,51 compounds were the major variables among different parts of E.phaseoloides.Additionally,seed kernels of E.phaseoloides displayed more dramatic antipruritic effect.And both seed kernels and stems exhibited marked analge-sic activity.Moreover,according to the results of correlation analysis,4 compounds,including epigallocatechin-3-O-gallate(51)and cynaroside(64),contributed significantly to antipruritic effect while 11 compounds containing eriodictyol(5),3',4',7-trihydroxy-flavone(11),3-O-[β-D-xylopyranosyl-(1→2)-α-L-arabinopyranosyl-(1→6)]-2-acetylamino-2-deoxy-β-D-glucopyranosylenta-genic acid(20)and cynaroside(64)were associated with analgesic activities.CONCLUSION This study has comprehensively compared the chemical constituents in different parts of E.phaseoloides and evaluated the antipruritic and analgesic activities.And the flavonoids and triterpenoids in E.phaseoloides are associated with pharmacological properties.
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