Chemical profiling and tissue distribution of Shenshuai Yifang after oral dosing in rats by UHPLC-Q-Exactive-Orbitrap HRMS
OBJECTIVE To employ UHPLC-Q-Exactive-Orbitrap HRMS to identify the chemical components of Shensh-uai Yifang and explore the distribution of major components in blood and tissues after oral dosing in rats.METHODS An Acquity UPLC BEH C18 chromatographic column(100 mm×2.1 mm,1.7 μm)was utilized as solid phase while mobile phase composed of 0.1％formic acid(A)and methanol(B)with gradient elution.Mass spectra were collected with a Full scan of posi-tive and negative ions/data-dependent on secondary scan function(Full MS/dd-MS2)for data collection.Chromatographic reten-tion time and quasi-molecular ion fragment information were acquired by Xcalibur 4.2 software while identification of components was compared with reference standards,literature reference and self-established database.RESULTS A total of 166 chemical components were identified from Shenshuai Yifang,including 56 kinds of flavonoids,25 kinds of saponins,24 kinds of anthraqui-nones,23 kinds of organic and phenolic acids,14 kinds of phthalides,7 kinds of cyclic peptides and alkaloids,3 kinds of lignans and coumarins and 4 kinds of others.After oral dosing of Shenshuai Yifang to rats,54,53,61,47,71,48,53,42 prototypic components were detected in sera,heart,liver,spleen,lung,kidney,brain and testicles respectively.CONCLUSION In this study,the chemical components and major blood/tissue distribution components of Shenshuai Yifang,have been rapidly identified by high-resolution mass spectrometry.It may provide references for analyzing effective substances and elucidating the action mechanism of Shenshuai Yifang.
UHPLC-Q-Exactive Orbitrap HRMSShenshuai Yifangchemical compositionabsorded componentstissue distribution
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