Therapeutic material basis of stir-fried Tartary buckwheat in treating diabetes mellitus based upon LC-MS plus network pharmacology
OBJECTIVE To explore the different components of Tartary buckwheat before and after roasting and elucidate its mechanism of action in treating diabetes mellitus(DM).METHODS The chemical compositions of Tartary buckwheat were identified by ultra-high performance liquid chromatogram-quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS).And chemical pattern recognition technique was utilized for screening different components of Tartary buckwheat before and after roasting.Different component targets before and after stir-frying were mapped to diabetic targets by network pharmacology method.Component-target interaction network was constructed and core target genes were screened for GO and KEGG enrich-ment analysis.RESULTS A total of 16 kinds of characteristic components of sauteed buckwheat were identified.And 518 cross-over target genes and 167 signaling pathways were implicated.Quercetin,kaempferol,erucic acid and alfalfa were key active com-ponents while SRC,MAPK3 and MAPK1 were potential targets.CONCLUSION Stir-fried buckwheat may alleviate DM through various signaling pathways,providing theoretical rationales for its anti-diabetic application.
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