岗梅清咽合剂HPLC特征图谱及同时测定多成分含量
HPLC characteristic chromatogram of Gangmei Qingyan Mixture and simultaneous determination of multiple components
林秀娟 1刘翠珍 1雷晓椿 1曾德浩 2丘振文 3李得堂3
作者信息
- 1. 广州中医药大学第一临床医学院,广东 广州 510405
- 2. 广州中医药大学第一附属医院药学部,广东 广州 510405
- 3. 广州中医药大学第一临床医学院,广东 广州 510405;广州中医药大学第一附属医院药学部,广东 广州 510405;广东省中医临床研究院,广东 广州 510405
- 折叠
摘要
目的:建立岗梅清咽合剂的高效液相色谱(HPLC)特征图谱,以及同时测定多个特征性成分含量.方法:采用HPLC法建立10批岗梅清咽合剂的特征图谱,评价其相似度,确定共有特征峰,并对各共有峰进行归属;以特征图谱共有峰峰面积为分析对象,进行化学计量法分析,根据变量重要性投影(VIP)值大于1,筛选出差异性成分,并对6个特征性化学成分同时测定其含量.结果:10批岗梅清咽合剂共标定出21个共有特征峰,10批样品的相似度均大于0.950,与对照品比对出了 11个特征峰.CA和PCA结果显示,10批样品可聚为3类;OPLS-DA结果显示,VIP值大于1的成分筛选出了 10个成分,依次为4(没食子酸),10、9(咖啡酸),13、8(隐绿原酸),2、3、17(异绿原酸C),15(异绿原酸A),7(绿原酸)号峰.10批样品中没食子酸、绿原酸、隐绿原酸、咖啡酸、异绿原酸A、异绿原酸C的含量分别为0.097～0.591、0.055～0.131、0.051～0.200、0.402～1.171、0.013～0.087、0.013～0.123 mg·mL-1.结论:建立的HPLC特征图谱方法稳定可行,可用于岗梅清咽合剂的质量控制.
Abstract
OBJECTIVE To establish the characteristic chromatogram of Gangmei Qingyan Mixture(GQM)with high per formance liquid chromatography(HPLC)and simultaneous content determination of multiple characteristic components.METHODS HPLC was utilized for establishing the characteristic atlas of 10 batches of GQM,evaluating their similarity,deter-mining the common characteristic peaks and assigning the common peaks.Stoichiometric analysis was performed through taking the common peak area of characteristic map as a variable.The differential components were screened out according to the impor-tance projection(VIP)value of variable＞1.And the contents of 6 characteristic chemical components were determined simulta-neously.RESULTS A total of 21 common characteristic peaks were identified in 10 batches of GQM.The similarity of 10 prepa-ration batches was＞0.950 and 11 characteristic peaks were detected when compared with control products.The results of cluster analysis(CA)and principal component analysis(PCA)revealed that 10 batches of samples could be classified into three distinct categories.The results of orthogonal partial least squares discriminant analysis(OPLS-DA)indicated that 10 components were screened out for components with VIP value＞1,namely 4(gallic acid),10/9(caffeic acid),13/8(cryptochlorogenic acid),2/3/17(isochlorogenic acid C),15(isochlorogenic acid A)and 7(chlorogenic acid).The contents of gallic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,isochlorogenic acid A and isochlorogenic acid C in 10 batches of samples were(0.097-0.591),(0.055-0.131),(0.051-0.20),(0.402-1.171),(0.019-0.104)and(0.013-0.123)mg·mL-1.CONCLUSION Both stable and feasible,HPLC characteristic mapping may be employed for quality control of GQM.
关键词
岗梅清咽合剂/特征图谱/化学计量法/含量测定Key words
Gangmei Qingyan Mixture/feature map/stoichiometry/content determination引用本文复制引用
出版年
2024
NETL
NSTL
万方数据