HPLC characteristic chromatogram of Gangmei Qingyan Mixture and simultaneous determination of multiple components
OBJECTIVE To establish the characteristic chromatogram of Gangmei Qingyan Mixture(GQM)with high per formance liquid chromatography(HPLC)and simultaneous content determination of multiple characteristic components.METHODS HPLC was utilized for establishing the characteristic atlas of 10 batches of GQM,evaluating their similarity,deter-mining the common characteristic peaks and assigning the common peaks.Stoichiometric analysis was performed through taking the common peak area of characteristic map as a variable.The differential components were screened out according to the impor-tance projection(VIP)value of variable＞1.And the contents of 6 characteristic chemical components were determined simulta-neously.RESULTS A total of 21 common characteristic peaks were identified in 10 batches of GQM.The similarity of 10 prepa-ration batches was＞0.950 and 11 characteristic peaks were detected when compared with control products.The results of cluster analysis(CA)and principal component analysis(PCA)revealed that 10 batches of samples could be classified into three distinct categories.The results of orthogonal partial least squares discriminant analysis(OPLS-DA)indicated that 10 components were screened out for components with VIP value＞1,namely 4(gallic acid),10/9(caffeic acid),13/8(cryptochlorogenic acid),2/3/17(isochlorogenic acid C),15(isochlorogenic acid A)and 7(chlorogenic acid).The contents of gallic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,isochlorogenic acid A and isochlorogenic acid C in 10 batches of samples were(0.097-0.591),(0.055-0.131),(0.051-0.20),(0.402-1.171),(0.019-0.104)and(0.013-0.123)mg·mL-1.CONCLUSION Both stable and feasible,HPLC characteristic mapping may be employed for quality control of GQM.
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