Fistular onion bulb regulated HIF-1α/VEGF pathway for arresting oxidative stress and apoptosis of hypoxic/reoxygenated cardiomyocytes
OBJECTIVE To evaluate the effects of fistular onion bulb(FOB)extract on oxidative stress and apoptosis in hypoxia/reoxygenated H9c2 cardiomyocytes through regulating HIF-1α/VEGF pathway.METHODS Hypoxia/reoxygenation(H/R)cell model was constructed by H9c2 rat cardiomyocytes with a reoxygenation time of 24 h as determined by cell morphol-ogy observation and CCK-8 assay.The cells were assigned into five groups of control,H/R,H/R+FOB,H/R+YC-1(HIF-1α inhibitor)and H/R+YC-1+FOB.Cell proliferation activity was assessed by CCK-8 while cell apoptosis by flow cytometry.The contents of reactive oxygen species(ROS)were detected by flow cytometry and malondialdehyde(MDA)and level of lac-tate dehydrogenase(LDH)by biochemical kits.Westem blot was utilized for determining the protein expression levels of Bax,Bcl-2,hypoxia-inducible factor-1α(HIF-1α)and vascular endothelial growth factor(VEGF).RESULTS As compared with control group,cell proliferation activity and apoptotic rate decreased in H/R group(P＜0.05)while the contents of ROS,MDA and LDH rose significantly(P＜0.05).Additionally,apoptosis-related protein Bax was up-regulated(P＜0.05)while Bcl-2 down-regulated(P＜0.05).Protein expression levels of HIF-1α/VEGF pathway became elevated(P＜0.05).As compared with H/R group,cell proliferation activity spiked obviously in both H/R+FOB and H/R+YC-1 groups(P＜0.05)while apop-totic rate decreased(P＜0.05).Furthermore,the contents of ROS,MDA and LDH all declined(P＜0.05)while Bcl-2 protein was up-regulated(P＜0.05).The protein expressions of Bax,HIF-1α and VEGF all decreased(P＜0.05).CONCLUSION FOB may suppress the activation of HIF-1α/VEGF pathway,resulting in lower oxidative stress and slower apoptosis within hypoxic/reoxygenated cardiomyocytes,ultimately alleviating cellular injury.
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