Optimization of extraction process of Danggui BuxueTangand its protective effect on cerebral ischemia/reperfusion injury model rats
OBJECTIVE To optimize the extraction technology of Danggui Buxue Tang(DBT)and examine its improve-ment effect on the symptom of hemorrhoid model rats.METHODS The contents of astragaloside Ⅳ,calycosin,ferulic acid,calycosin-7-O-β-D-glucoside,formononetin and cis-ligustilidesix components were employed as evaluation parameters.Weight coefficient of each component was evaluated by entropy weight method-AHP.The effects of solvent concentration,solvent vol-ume and extraction time on the extraction process of DBT were investigated by multi-index comprehensive scoring combined with Box-Behnken response surface method.The middle cerebral artery occlusion/reperfusion(MCAO/R)model of rats was utilized for evaluating protective effect of the new technology of Danggui Buxue lyophilized powder on cerebral ischemia/reperfusion injury.RESULTS The optimal extraction conditions were ethanol concentration 86％,a ratio of liquid-material at 10∶1(mL∶g),extracted twice,2.5 h each time,filtered by No.10 sieve,concentrated under reduced pressure(＜50 ℃)and freeze-dried into freeze-dried powder.Pharmacodynamics results showed that Danggui Buxue freeze-dried powder could improve the neurological deficit,shrink the area of cerebral infarction,alleviate the histopathological damage,reduce the contents of tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)and malondialdehyde(MDA)in brain tissue of rats(P＜0.05)and boost the activities of CAT and SOD(P＜0.05).CONCLUSION The extraction process of DBT is scientific,reasonable,stable and feasible.The new technology of Danggui Buxue Freeze-dried Powder obtained by this process can improve cerebral ischemia/reperfusion injury in rats and provide rationales for follow-up studies of the new preparation.
NETL
NSTL
万方数据
