Establishment of fingerprints and spectrum-effect relationship of anti-fatigue and immunoregulatory activity of Lycium barbarum L polysaccharide
OBJECTIVE To explore the correlation between the fingerprint of Lycium barbarum polysaccharide(LBP),examine its effects on anti-fatigue and immune regulation and elucidate the pharmacodynamic material basis for its anti-fatigue and immune-modulating properties.METHODS Samples were prepared from 10 batches of wolfberry sourced from various origins for chemical fingerprinting.Cluster analysis,principal component analysis and partial least squares regression analysis were employed for analyzing common peaks in these fingerprints with reference substances utilized for identification.Anti-fatigue effect of LBP was assessed by such parameters as murine swimming time,blood urea nitrogen(BUN),lactic acid(LA),liver glycogen and lactate dehydrogenase(LDH).Additionally,an immunosuppressive murine model was established through an intraperitoneal injection of cyclophosphamide.Splenic index,thymus index,tumor necrosis factor-alpha(TNF-α),interferon-α/interferon-β(IFN-α/IFN-β),interleukin-2(IL-2),interleukin-6(IL-6),immunoglobulin A(IgA),immunoglobulin G(IgG),delayed-type hypersensitivity(DTH)and median hemvlytic dose(HD50)were employed as parameters for evaluating the immunomodula-tory activity of LBP.Finally spectrum-effect relationship was examined through gray correlation analysis.RESULTS The estab-lished fingerprint identified 21 common peaks and confirmed 5 common peaks;fingerprint similarity surpassed 0.741 and principal components divided samples from different origins into 5 categories.It was the same as the cluster analysis results;gray correla-tion results were anti-fatigue.The first six chromatographic peaks were 2,6,7,12,15 and 17 respectively.And the first six chro-matographic peaks of immune regulation were 4,6,7,9,12 and 17 respectively;2,5,6,7,15 and chromatographic peak No.21 were correlated positively with anti-fatigue drug effect.And chromatographic peaks No.2,4,7,8,11,12,13 and 21 were correlated positively with immunomodulatory drug effect.CONCLUSION Anti-fatigue and immunomodulatory effects of wolf-berry arise from the synergistic action of multiple components.Chromatographic peaks 2,6,7 and 15 are likely the primary active ingredients contributing to anti-fatigue effects of LBP while chromatographic peaks 4,7 and 12 may represent the major active ingredients involved in immune regulatory functions of LBP.
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