目的：建立木香药材高效液相色谱特征图谱分析方法。方法：采用Diamonsil C18色谱柱（250 mm ×4．6 mm，5μm），以甲醇（ A）－0．1％甲酸水（ B）为流动相梯度洗脱，流速1．0 ml／min，检测波长254 nm，柱温25℃，建立不同产地木香的高效液相色谱特征图谱；采用《中药色谱指纹图谱相似度评价系统》（2004 A版）进行相似度评价。结果：通过对15批次不同产地木香样品的分析，建立了木香药材高效液相色谱特征图谱，确定了6个共有特征峰，并指认了其中2个特征峰的结构，分别为木香烃内酯和去氢木香内酯。结论：本研究建立的木香药材特征图谱的特征性和专属性强，且方法简便、可靠，重复性好，为更好地控制木香药材的内在质量提供了科学依据。
Study on Characteristic Fingerprint of Aucklandia Radix by HPLCΔ
OBJECTIVE:To establish the HPLC characteristic fingerprint for the quality control of Aucklandia Radix. METHODS:Diamonsil C18 chromatographic column(250 mm ×4.6 mm, 5μm) was adopted, the mobile phase consists of methanol(A) and water containing 0.1%acetic acid(B) with gradient elution at the flow rate of 1.0 ml/min.The UV detection wavelength was 254 nm and the column temperature was 25 ℃.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine ( version 2004A) was used to calculate the similarity of the HPLC characteristic fingerprint of Aucklandia Radix from different habitats.RESULTS:HPLC characteristic fingerprint was established with six common characteristic peaks based on the analysis of 15 batches of Aucklandia Radix.Two common characteristic peaks were identified as costunolide and dehydrocostus lactone.CONCLUSIONS:The established method shows good characteristics, specificity, and repeatability, which can provide a scientific basis for the identification and quality control of Aucklandia Radix.
Aucklandia RadixCharacteristic fingerprintHPLCQuality control
国家科技期刊平台
NSTL
万方数据
维普
