OBJECTIVE:To investigate the antidepressant effects and mechanism of Jinaotong(JNT)with extracts of sea buckthorn and peanut hull as its main components.METHODS:(1)At the animal level,chronic unpredictable mild stress(CUMS)depression model mice were randomly divided into the blank group,model group,JNT administration group(JNT low concentration group and JNT high concentration group)and fluoxetine group.The antidepressant effects of JNT were inspected by behavioral studies such as body weight test,sugar-water preference test and tail suspension immobility time test.(2)At the cellular level,different concentrations of JNT were applied to PC 12 cells,SH-SY5Y cells,BV2 cells and RAW 264.7 cells,so as to probe into the promotional effects of JNT on the function of neurotrophic factors,the protection of neuronal cells and the induction of neural differentiation,as well as the antioxidant and anti-inflammatory effects.RESULTS:(1)Compared with the blank group,the sugar-water preference rate and body weight of the model group decreased significantly,the tail suspension immobility time and forced swimming immobility time of the model group were significantly prolonged(P＜0.01).The sugar-water preference rate of JNT administration group was significantly higher than that of the model group(P＜0.05),the body weight of the JNT administration group and fluoxetine group were significantly higher than that of the model group(P＜0.01),the tail suspension immobility times of the JNT low concentration group and JNT high concentration group were significantly shorter than that of the model group(P＜0.01,P＜0.05),the forced swimming immobility time of the JNT administration group was significantly shorter than those of the model group(P＜0.01),the differences were statistically significant.(2)When JNT was synergistically administered with nerve growth factor(5 and 50 ng/mL),the differentiated degree of PC 12 cell and activity of pNF200-Luc expression luciferase increased significantly.The luciferase activity increased significantly when JNT was synergistically administered with brain-derived neurotrophic factor,neurotrophin-3 and neurotrophin-4(5 ng/mL).Dose from 100 to 1 000 μg/mL of JNT could significantly reduced amyloid β-protein aggregation.After the oxidative damage of PC 12 cells induced by tBHP,the application of JNT can increased the activity of PC12 cells,the higher the concentration,the better the effect.After the iron death of SH-SY5Y cells induced by Erastin,the application of JNT can increase the activity of SH-SY5Y cells,the higher the concentration,the better the effect.After using lipopolysaccharide on BV2 and RAW 264.7 cells,the application of JNT can reduce the mRNA level of interleukin-1 β and the nuclear factor kappa B transcriptional activity,which showed a concentration-dependent manner.CONCLUSIONS:JNT has the effects of anti-inflammatory,antioxidant,protecting nerve cells,inducing the expression of neurofilaments,and enhancing the neurotrophic factor-like;the JNT exerts antidepressant effects through the above effects.
Extracts of sea buckthorn and peanut hullJinaotongAntidepressant
万方数据
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