Mechanism of Yijin Formula in the Treatment of Lung Cancer Based on Network Pharmacology
OBJECTIVE:To probe into the mechanism of Yijin formula in the treatment of lung cancer based on previous research results,network pharmacology and molecular docking technology.METHODS:Yijin formula drug-acting genes and lung cancer-related genes were obtained from multiple open data platforms.Drug component-target correlation and key compounds were explored based on Cytoscape.Protein-protein interaction(PPI)network was established based on STRING platform,and Cytoscape-CytoNCA topological analysis was used to screen the core targets.Gene ontology(GO)and Kyoto gene and genome encyclopedia analysis of targets were performed by R-ClusterProfiler.Finally,AutoDock was used to validate the docking of key active components with core targets related to lung cancer pathways.RESULTS:The active components of Yijin formula were 157,which contained 235 potential therapeutic targets.A total of 8 585 lung cancer-related targets were obtained from 5 data platforms.The number of potential therapeutic targets shared by the drug and the data platforms was 215.A total of 18 core targets were screened from the PPI core network.GO functional enrichment analysis showed that the related targets were significantly enriched in various biological processes and cell functions,and the targets were closely related to lung cancer signaling pathways.According to molecular docking results,paclitaxel,quercetin,naringenin,lignocerotoxin,glycyrrhizin chalcone A,kaempferol,isorhamnetin,diosgenin element and aconitine were able to bind tightly to the core targets associated with the lung cancer pathway.CONCLUSIONS:The active components of Yijin formula may intervene in lung cancer through multiple targets and signaling pathways,which may be one of the mechanisms of therapeutic effects.
Piao BingkuiLung cancerNetwork pharmacologyMolecular dockingYijin formula
万方数据
维普
