合欢树不同部位挥发油成分及其气相色谱-四极杆飞行时间串联质谱分析
Gas Chromatography-Quadrupole Time-of-Flight Mass Spectrometry Analysis on Chemical Components of Volatile Oil from Different Parts of Albizia Julibrissin Durazz
冯欣 1刘敏 1田佳懿 1陈丽芳 1王林燕 2王淑梅1
作者信息
- 1. 首都医科大学附属北京世纪坛医院药学部,北京 100038
- 2. 浙江中医药大学中医药科学院,杭州 310053
- 折叠
摘要
目的:分析合欢树不同药用部位(合欢花、合欢米及合欢皮)中的挥发性成分及其相对含量.方法:采用同时蒸馏-萃取方法提取合欢花、合欢米及合欢皮的挥发油,并运用气相色谱-四极杆飞行时间串联质谱(GC-Q/TOF-MS)技术对其化学成分进行分析,通过NIST 14.L数据库进行化合物检索与鉴别,采用面积归一法确定各成分的相对含量.结果:从合欢花、合欢米及合欢皮挥发油中分别鉴定出主要成分 80、61 及 42 种,分别占其挥发性成分总量的 96.44%、97.67%及 93.36%.合欢皮的脂肪族化合物相对百分含量高于合欢米、合欢花;合欢皮的芳香族化合物、萜类化合物相对百分含量与数量明显低于合欢花和合欢米.合欢花、合欢米及合欢皮三者共有成分 11 种;合欢花特有成分 25 种,合欢米特有成分 10 种,合欢皮特有成分 27 种.结论:本研究采用GC-Q/TOF-MS技术对合欢花、合欢米及合欢皮中的挥发性成分进行比较分析,可为植物合欢的合理开发和利用提供科学依据.
Abstract
OBJECTIVE:To analyze the volatile components in different medicinal parts of Albizia Julibrissin Durazz(albiziae flos,Hehuanmi and silktree albizziae)and the relative contents.METHODS:Volatile oil from albiziae flos,Hehuanmi and silktree albizziae was extracted by simultaneous distillation extraction method.Chemical constituents were determined by gas chromatography-quadrupole time-of-flight mass spectrometry(GC-Q/TOF-MS).Compounds were searched and identified through the NIST 14.L database.The relative content of each component was determined by area normalization.RESULTS:A total of 80,61 and 42 components were identified from the volatile oil of albiziae flos,Hehuanmi and silktree albizziae,accounting for 96.44%,97.67%and 93.36%of the total volatile components.The relative percentage of aliphatic compounds in silktree albizziae was higher than that in albiziae flos and Hehuanmi.The relative percentage and quantity of aromatic compounds and terpenoids in silktree albizziae were significantly lower than those in albiziae flos and Hehuanmi.There were 11 components shared by albiziae flos,Hehuanmi and silktree albizziae.There were 25 unique components of albiziae flos,10 unique components of Hehuanmi,and 27 components of silktree albizziae.CONCLUSIONS:GC-Q/TOF-MS technique can be used to analyze and compare the volatile components of albiziae flos,Hehuanmi and silktree albizziae,which provides scientific basis for the rational development and application.
关键词
合欢树不同部位/挥发油/同时蒸馏-萃取/气相色谱-四极杆飞行时间串联质谱Key words
Different parts of Albizia Julibrissin Durazz/Volatile oil/Simultaneous distillation extraction/Gas chromatography-quadrupoletime-of-flight mass spectrometry引用本文复制引用
基金项目
国家自然科学基金资助项目(81872926)
北京市属医院科研培育计划项目(PZ2019008)
出版年
2024
国家科技期刊平台
NSTL
万方数据