Medication Rules and Mechanism of Non-alcoholic Fatty Liver Disease from Sputum Dampness
OBJECTIVE:To explore the prescription rules of non-alcoholic fatty liver disease(NAFLD)from sputum dampness,and analyze the potential mechanism of core prescription drugs.METHODS:Clinical research literature on the treatment of NAFLD from phlegm dampness was collected,and systematic clustering and association rules analysis were carried out on taste and attribution of prescription drugs.After determining the core prescription for treating NAFLD from the perspective of phlegm-dampness theory,we searched the database to obtain the drug's effective ingredient targets and disease targets.The intersection of these targets was used to construct a protein-protein interaction network in the STRING database.Subsequently,all intersecting targets were scored in Cytoscape to identify core targets.Gene Ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were conducted on key targets.The analysis results were imported into Cytoscape to construct a network diagram of active ingredients-targets-disease pathways for traditional Chinese medicine.RESULTS:After screening,a total of 119 prescriptions were obtained,including 153 kinds of drugs,which were sweet and warm,and were more common in the liver and spleen.Analysis of association rules showed that Crataegi Folium,Wolfiporia Cocos,Alisma Orientale,Atractylodes Macrocephala Koidz,Radix Salviae,Arum Ternatum Thunb and Citrus Reticulata were the core drugs for the treatment of NAFLD.Core prescription combination of"Crataegi Folium,Cassiae Semen,Alisma Orientale,Radix Salviae,Radix Bupleuri and Curcumae Radix"was obtained by cluster analysis.Network pharmacological analysis showed that there were 248 intersection targets between the core prescription and NAFLD,and the signal transduction and transcriptional activator 3 and myelocytomatosis oncogene cellular homolog were the core targets for the treatment of NAFLD from sputum wetness.Forkhead box O,tumor necrosis factor and phosphatidylinositol 3 kinase-protein kinase B signaling pathways were the main signaling pathways of phlegm-dampness treatment of NAFLD.Methyl heptenone,quercetin and kaempferol were the main effective components of the core prescription.CONCLUSIONS:The treatment of NAFLD from phlegm dampness is mainly sweet and warm medicine,which is inseparable from the regulation of liver and spleen,and Crataegi Folium,Wolfiporia Cocos,Alisma Orientale,Atractylodes Macrocephala Koidz,Radix Salviae,Arum Ternatum Thunb and Citrus Reticulata are commonly used drugs for the treatment of NAFLD from phlegm dampness,and the core prescription of NAFLD from phlegm dampness mainly plays a therapeutic role in NAFLD by participating into the regulation of lipid metabolism,inflammatory response,oxidative stress and other biological processes.
万方数据
维普
