Mechanisms of Radix Bupleuri-Radix Paeoniae Alba in the Treatment of Cervical High-Risk Human Papilloma Virus Infection Base on Network Pharmacology
OBJECTIVE:To probe into the core drug pairs of traditional Chinese medicine(TCM)in the treatment of cervical high-risk human papilloma virus(HPV)infections by using data mining methods,and to clarify the mechanism of the core drug pairs by network pharmacology methods.METHODS:Related literature were retrieved from CNKI,Wanfang Data and VIP database to screen out core drug pairs.The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was retrieved for the relevant active ingredients and targets of core drug pairs;the On-Line Mendelian Inheritance in Man and GeneCards databases were retrieved for disease-related targets.Cytoscape 3.9.1 software was used to construct core targets network.DAVID database was used to perform gene ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis on the targets.RESULTS:A total of 216 studies,209 prescriptions and 194 kinds of traditional Chinese medicine were enrolled,the core drug pair of Radix bupleuri-Radix paeoniae aba was obtained.A total of 15 active ingredients and 433 targets in Radix bupleuri as well as 9 active ingredients and 161 targets in Radix paeoniae alba were screened out,and 297 targets remained after deduplication;there were 116 intersection targets with disease.The core targets of Radix bupleuri-Radix paeoniae alba for the treatment of cervical high-risk HPV infection included protein kinase B(Akt)1,tumour necrosis factor,interleukin 6 and PPARG.The mechanism of action was mainly exerted through the cancer pathway,phosphatidylinositol 3 kinase-Akt signaling pathway,etc.;the biological processes involved mainly included the positive regulation of gene expression and the negative regulation of apoptotic process,etc.CONCLUSIONS:This study preliminarily verifies that Radix bupleuri-Radix paeoniae alba can participate into the inflammatory response and immune regulation through multi-targets and multi-pathways,so as to achieve the therapeutic effect of cervical high-risk HPV infection.
Cervical high-risk human papilloma virus infectionRadix bupleuriRadix paeoniae albaNetwork pharmacologyMechanism
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