Mechanism of Angelicae Sinensis Radix-Chuanxiong Rhizoma in the Treatment of Psoriasis Based on Network Pharmacology and Molecular Docking
OBJECTIVE:To predict the potential molecular mechanism of Angelicae sinensis radix-Chuanxiong rhizoma in the treatment of psoriasis based on network pharmacology and molecular docking techniques.METHODS:Active components and specific functional targets of Angelicae sinensis radix-Chuanxiong rhizoma were screened out by using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,psoriasis-related targets were obtained by retrieving GeneCard,OMIM,DrugBank and DisGeNET databases.The drug-disease common targets were acquired by using Venn web tools,which were imported into Cytoscape 3.9.1 software to map Angelicae sinensis radix-Chuanxiong rhizoma-common targets-psoriasis network diagram.The protein-protein interaction network was constructed by STRING database and Cytoscape 3.9.1 software,the Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed by DAVID database,the bubble diagram of enrichment analysis was made through bioinformatics.Molecular docking was verified by AutoDock software,and PyMOL software was using to conduct visualization on molecular docking.RESULTS:A total of 9 active ingredients and 28 potential targets were obtained from Angelicae sinensis radix-Chuanxiong rhizoma for the treatment of psoriasis,the key targets were Cystatinase 3,cyclooxygenase 2,estrogen receptor 1,transcription factor AP-1 and mitogen-activated protein kinase 14.Which mainly involved the drug-responsive signaling pathway,intracellular steroid hormone receptor signaling pathway,phosphatidylinositol 3-kinase-protein kinase B signaling pathway and interleukin 17 signaling pathway.CONCLUSIONS:Angelicae sinensis radix-Chuanxiong rhizoma realizes the treatment of psoriasis through multiple pathways,multiple active components and multiple effective targets.
万方数据
维普
