Mechanism of Fufang Yizhihuanghua Spray in the Treatment of Upper Respiratory Tract Infection Based on Network Pharmacology and Molecular Docking
OBJECTIVE:To probe into the mechanism of Fufang Yizhihuanghua spray in the treatment of upper respiratory tract infection.METHODS:Components of Fufang Yizhihuanghua spray were selected by using Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.The targets were predicted in Swiss Target Prediction database.Combined with the upper respiratory tract infection-related targets obtained from GeneCards and other databases,the intersection targets and active components were imported into Cytoscape 3.9.0 software to obtain the interaction network of component-potential target-disease.Protein-protein interaction(PPI)analysis of intersection targets was performed by using STRING database.Key targets were selected for gene ontology functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.AutoDockTools 1.5.7 software was used to predict the binding activity of core components and core targets with high degree ranking.RESULTS:Totally 235 gene targets were obtained after the intersection of the relevant component targets and disease targets of FufangYizhihuanghua spray as potential targets for the treatment of upper respiratory tract infection.PPI and Topology analysis identified 95 key targets.KEGG enrichment analysis showed that the pathway was mainly concentrated in the PI3K-Akt signaling pathway.Molecular docking results showed that the core components such as quercetin and luteolin,can stably bind to the core targets such as PTGS2,Akt1,and CASP3.CONCLUSIONS:The results predicts and verifies the mechanism of Fufang Yizhihuanghua spray in the treatment of upper respiratory tract infection,and lays foundation for further research.
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