电针对动脉粥样硬化兔腹腔巨噬细胞受体CD36表达的影响
Effects of electroacupuncture on expression of CD36 in peritoneal macrophages of rabbits with atherosclerosis
成泽东 1宁玉楼 1王芮1
作者信息
- 1. 辽宁中医药大学针灸推拿学院,沈阳 110847
- 折叠
摘要
目的:观察电针对动脉粥样硬化兔腹腔巨噬细胞受体CD36基因和蛋白表达的影响,探讨针刺调节动脉粥样硬化的作用机制.方法:将26只雄性新西兰兔随机分为空白组(7只)和造模组(19只).空白组普通饲料喂养,造模组采用高脂饲料喂养加颈总动脉球囊损伤术复制动脉粥样硬化模型,8周后,随机从空白组和造模组中各取1只兔处死,采用HE染色观察兔颈动脉粥样硬化斑块病理形态学变化确定是否造模成功.造模组再随机分为模型组、电针组和西药组,每组6只.空白组普通饲料喂养,其余组高脂饲料喂养.空白组和模型组不加干预因素,同电针组抓取、固定;电针组针刺双侧"内关""足三里"及"关元"穴,双侧"内关""足三里"穴连接电针仪20 min,采用疏密波(4 Hz/20 Hz,1 mA);西药组给予阿托伐他汀钙片(1 mg/kg/d)混悬液20 mL灌胃给药,两组均每天治疗1次,6 d为一疗程,疗程间休息1 d,共治疗4个疗程.采用蛋白质免疫印迹法检测腹腔巨噬细胞受体CD36蛋白表达,采用RNA的反转录(RT)和cDNA的聚合酶链式扩增反应(PCR)相结合的技术检测腹腔巨噬细胞受体CD36m RNA表达.结果:空白组血管壁厚度较均一,内皮较完整,未见泡沫细胞的堆积以及粥样斑块;造模组动脉内膜明显增厚,内膜受损,内膜下可见明显的粥样斑块,炎性细胞浸润,可见泡沫细胞.干预后模型组、电针组和西药组腹腔巨噬细胞受体CD36蛋白、mRNA表达均明显高于空白组(均P<0.01),电针组和西药组腹腔巨噬细胞受体CD36蛋白、mRNA表达均明显低于模型组(均P<0.01),电针组腹腔巨噬细胞受体CD36蛋白、mRNA水平表达与西药组比较差异无统计学意义(均P>0.05).结论:电针可以降低动脉粥样硬化兔腹腔巨噬细胞受体CD36蛋白及mRNA表达水平,这可能是电针治疗动脉粥样硬化的作用机制之一.
Abstract
Objective To observe the effect of electroacupuncture (EA) on the expressions of CD36 gene and protein in peritoneal macrophages of atherosclerotic rabbits, and to explore the mechanism of acupuncture regulation of atherosclerosis.Methods A total of 26 male New Zealand rabbits were randomly divided into a blank group (7 rabbits) and a model group (19 rabbits). The rabbits in the blank group were fed with common feedstuff, and those in the model group were applied with high fat diet and arteria carotis communis bullon injury. One rabbit was sacrificed after 8 weeks to observe the morphological changes of carotid atherosclerotic plaques by HE staining to verify model establishment separately in the blank group and model group. The model rabbits were randomized into a control group, an EA group and a western medication group, 6 rabbits in each one. Common feedstuff was used in the blank group and high fat feed in the other groups. No intervention except grabbing and fixation as the EA group was in the blank group and control group. Rabbits in the EA group were treated with acupuncture at bilateral "Neiguan" (PC 6), "Zusanli" (ST 36), "Guanyuan" (CV 4), and EA with sparse-dense wave was connected at bilateral "Neiguan" (PC 6) and "Zusanli" (ST 36) for 20 min, 4 Hz/20 Hz and 1 mA. 20 mL suspension of 1mg/kg/d atorvastatin calcium tablets were prescribed by intragastric administration in the western medication group for 4 courses,6 d as one course with 1 d between two courses, once a day. The expression of CD36 protein in peritoneal macrophages was detected by Western blot. Reverse transcription (RT) of RNA and polymerase chain reaction (PCR) of cDNA were used to detect the expression of CD36 mRNA in peritoneal macrophages. Results In the blank group, the vascular wall thickness was uniform and the endothelium was intact. There was no accumulation of foam cells and atherosclerotic plaques. In the model group, the intima of the artery obviously thickened; the intima was damaged; the atherosclerotic plaque and inflammatory cell infiltration were found in the intima, and the foam cells were seen. After treatment, the expressions of CD36 protein and CD36 mRNA in the control group, EA group and western medication group were higher than those in the blank group (all P<0.01). Those in the EA group and western medication group were lower than the expressions in the control group (all P<0.01). There was no statistical significance for the expressions of CD36 protein and CD36 mRNA between the EA group and the western medication group (both P>0.05). Conclusion EA can reduce the expressions of CD36 protein and CD36 mRNA in peritoneal macrophages of atherosclerotic rabbits, which may be one of the mechanisms of EA treatment of atherosclerosis.
关键词
动脉粥样硬化/电针/穴,内关/穴,足三里/穴,关元/阿托伐他汀钙片/巨噬细胞/CD36Key words
atherosclerosis/electroacupuncture/Point PC 6 (Neiguan)/Point ST 36 (Zusanli)/Point CV 4 (Guanyuan)/atorvastatin calcium tablet/macrophages/CD36引用本文复制引用
基金项目
国家自然科学基金(81674078)
辽宁自然科学基金(201602479)
辽宁中医药大学中医脏象理论及应用教育部重点实验室开放基金(zyzx 1708)
出版年
2018
国家科技期刊平台
NSTL
维普
万方数据