The preliminary study on the effect of activation and inhibition of CDC42 gene on polar growth of Aspergil-lus fumigatus
Objective To construct CDC42 gene dominant activation and dominant inhibition point mutant strains and to explore the effects of the two point mutations on the polar growth and RAC1 gene expression of Aspergillus fumigatus.Methods CDC42 gene activation(CDC42G14V)and inhibition(CDC42T19N)strains were constructed by molecular biological methods,the characteristics of point mutation colonies were observed under light microscope,and the expression of RAC1 in Ku80,DA Cdc42 and DN Cdc42 strains was detected by RT-PCR.Results Point mutant strains of CDC42G14V and CDC42T19N were successfully constructed and further confirmed by sequencing.The colony growth diameters,conidia num-bers and spore germination rates of DA Cdc42 and DN Cdc42 were significantly different from those of the control strain Ku80(P＜0.05).The results of RT-PCR showed that the expressions of RAC1 in DA Cdc42 and DN Cdc42 strains were higher than that in the control strain Ku80(P＜0.05).Conclusions The activation and inhibition of CDC42 have negative effects on the polar growth of A.fumigatus and affect the expression of RAC1 gene related with polarity growth.
国家科技期刊平台
NSTL
万方数据
维普
