直接PCR与多重实时PCR检测临床标本的实用性评估
Evaluation of the practicability of direct PCR versus multiple real-time PCR for testing clinical specimens
臧晓慧 1梁官钊 1刘维达1
作者信息
- 1. 北京协和医学院/中国医学科学院/皮肤病医院/皮肤病研究所,南京 210042
- 折叠
摘要
目的 评估与传统方法相比,直接PCR与DG(Derma Genius)多重实时PCR在鉴定皮肤癣菌菌种的临床实用性.方法 收集2020年3月—2021年3月中国医学科学院皮肤病医院184例由临床医师怀疑浅部真菌感染的皮屑或甲屑标本,分别行显微镜检、培养、直接PCR和DG多重实时PCR.以镜检和培养为金标准,比较两种PCR方法鉴定皮肤癣菌的有效性.结果 直接PCR和DG-PCR的灵敏度分别为64％、99.2％,特异度分别为99.6％、88.1％,阳性预测值分别为97.5％、94.7％,阴性预测值分别为55.9％、98.1％.125例阳性标本中,培养出71例(56.8％),直接PCR检测出81例(64.8％),DG-PCR检测出99例(79.2％).结论 DG-PCR、直接PCR在检测皮肤癣菌感染方面有较高的实用价值.
Abstract
Objective To evaluate the clinical applicability of direct PCR and DG multiplex real-time PCR in the identifica-tion of dermatophytes strains compared with traditional methods.Methods A total of 184 samples of skin or nail scraps suspected of dermatophytosis were collected and divided into 4 samples.They were examined by direct microscopy,cul-tured,direct PCR and DG multiple real-time PCR,respectively.Using microscopic examination and culture as the gold standard,the efficacy of two skin PCR methods for identifying dermatomycosis was compared.Results The sensitivity of direct PCR and DG-PCR were 64％and 99.2％,respectively.The specificity was 99.6％and 88.1％,respectively.The positive predictive values were 97.5％and 94.7％,respectively.The negative predictive values were 55.9％and 98.1％,re-spectively.Among the 125 positive samples,71 cases(56.8％)were detected by culture,81 cases(64.8％)by direct PCR,and 99 cases(79.2％)by DG-PCR.Conclusion DG-PCR and direct PCR have high practical value in detecting dermatophy-ton infection.
关键词
皮肤癣菌病/PCR/DG-PCRKey words
dermatomycosis/PCR/DG-PCR引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据