Multicenter study of antifungal susceptibility of Trichosporon species causing invasive infection in China
Objective We aimed to investigate the antifungal susceptibility distribution of invasive Trichosporon infections in China.Methods A total of 133 non-duplicated Trichosporon isolates were collected from 43 medical centers which partic-ipate the CHIF-NET program during 7-year period,from August,2009 to July,2016.In vitro antifungal susceptibilities were performed using the Clinical and Laboratory Standards Institute(CLSI)broth microdilution method.Results A total of 10 species were identified in the multicenter invasive Trichosporon infections in China.T.asahii remains the leading pathogen(108/133 isolates),and blood is the most common type of specimen(36.8％).By IGS sequencing,five kinds of genotype were identified in T.asahii.Genotype 1(41.7％),genotype 4(31.5％)and genotype 3(23.1％)are the main genotypes in China.The overall distribution of MIC values of T.asahii to amphotericin B was higher than that of non-asahii Trichosporon isolates,with GM values of 1.36 μg/mL and 0.7 μg/mL,respectively.47.2％of T.asahii(51/108 isolates)have MIC values 2 μg/mL against amphotericin B,while non-asahii Trichosporon isolates(25 isolates)have MIC values＜1 μg/mL.High fluconazole MICs(≥ 8 μg/mL)were observed for 25％of T.asahii(27/108 isolates),2 i-solates of T.dohaense and each one isolate of T.asteroides,T.mucoides and T.faecale.Voriconazole was the most po-tent antifungal agent in vitro,with a GM of 0.09 μg/mL.The tentative ECV values for fluconazole,voriconazole,itracon-azole and posaconazole were 16μg/mL,0.25 μg/mL,1 μg/mL and 1 μg/mL,respectively.While the ECV values for am-photericin B and 5-flucytosine were 4 μg/mL.Conclusions The antifungal susceptibility distribution of invasive Trichos-poron infections were investigated in China.Then we established the tentative ECV values for T.asahii accordingly.
Trichosporon speciesantifungal susceptibilitygenotypeepidemiological cut-off value
万方数据
维普
