目的 以联苯苄唑溶液、盐酸特比萘芬溶液和苯扎氯铵原药稀释液为对照,检测美浮特皮肤抗菌液对耐药病原真菌的体外抑菌效果.方法 参考美国临床和实验室标准协会(Clinical and Laboratory Standards Institute,CLSI)的M38-A3和M27-A4方案,检测美浮特皮肤抗菌液对印度毛癣菌和红色毛癣菌抑制50％和90％生长所需的最低抑菌浓度(MIC);利用耳念珠菌、烟曲霉、不规则毛霉、尖端赛多孢和茄病镰刀菌等测试美浮特皮肤抗菌液抑制上述耐药真菌生长的MIC均值,评价过程中设置联苯苄唑溶液、盐酸特比萘芬溶液和苯扎氯铵稀释液作参照.结果 美浮特皮肤抗菌液对印度毛癣菌和红色毛癣菌的MIC50和MIC90均为1.76μg/mL(稀释1280倍),对比联苯苄唑溶液和盐酸特比萘芬溶液表现出较强的抑菌作用,且为苯扎氯铵稀释液MIC50和MIC90的1/4;耐药真菌中耳念珠菌的药物敏感浓度为<0.44μg/mL,体现美浮特皮肤抗菌液优质的抑菌效果,尖端赛多孢的GMIC值也低至0.88μg/mL,对烟曲霉在稀释640倍及以上时即可产生抑菌能力,而对不规则毛霉和茄病镰刀菌至少需要7μg/mL(即稀释320倍)才达到抑菌作用.结论 美浮特皮肤抗菌液对印度毛癣菌的抑菌浓度未因为该菌种耐药而比对照红色毛癣菌提高,体现其体外抗皮肤癣菌生长的优越性;对比联苯苄唑溶液和盐酸特比萘芬溶液,美浮特皮肤抗菌液对耐药真菌,包括固有耐药的耳念珠菌、茄病镰刀菌、尖端赛多孢等及获得性耐药的烟曲霉,均具有广泛且高效的体外抗菌抑菌效果,且优于对照苯扎氯铵稀释液,推荐今后可作为体外辅助途径为浅表真菌感染患者提供治疗思路,同时还可用于相关的环境病原真菌消杀和动物感染的浅表真菌防控.
In vitro sensitivity of drug-resistant pathogenic fungi using Meifute skin antimicrobial liquid
Objective The antifungal effect of Meifute skin antimicrobial liquid (benzalkonium chloride solution) against drug-resistant pathogenic fungi was tested in vitro with bifonazole solution and terbinafine hydrochloride solution as controls.Methords Referring to the CLSI guidelines of M38-A3 and M27-A4,using Trichophyton rubrum as a control,the mini-mum inhibitory concentrations (MICs) required for Meifute skin antimicrobial liquid to inhibit 50％ and 90％ growth of T.indotineae were tested.The GMICs of this solution against other drug-resistant fungi,including Candida auris,Aspergillus fumigatus,Mucor irregularis,Scedosporium apiospermum,Fusarium solani and so on were also measured.In both eval-uations,bifonazole solution,terbinafine hydrochloride solution and benzalkonium chloride solution were set as inhibitory ref-erences.Results The MIC50 and MIC90 of Meifute skin antimicrobial liquid against T.indotineae and T.rubrum were both 1.76 μg/mL (diluted 1280 times),which were only 1/4 concentration of benzalkonium chloride solution,demonstrating a strong antifungal effect compared to bifonazole solution and terbinafine hydrochloride solution.Among other drug-resistant fungi,C.auris showed the GMIC of Meifute skin antimicrobial liquid was less than 0.44 μg/mL,highlighting the excellent antifungal effect,while the GMIC value for S.apiospermum was as low as 0.88 μg/mL.A.fumigatus could exert antifun-gal capability at dilutions of 640 times and above,while M.irregularis and F.solani require at least 7 μg/mL (or a dilution of 320 times) for inhibition.Conclusion The inhibitory concentration of Meifute skin antimicrobial liquid against drug-re-sistant T.indotineae did not increase,compared with that of the control T.rubrum.Additionally,compared with bifon-azole solution and terbinafine hydrochloride solution,Meifute Skin antimicrobial liquid had extensive and highly efficient anti-fungal and inhibitory effects against drug-resistant strains,including C.auris,F.solani,S.apiospermum,and A.fu-migatus with theoutperformance of the control solution of benzalkonium chloride.It was recommended as a potential auxilia-ry treatment option for patients with superficial fungal infections in the future.Additionally,it can be used for the disinfec-tion of related environmental pathogenic fungi and the control of superficial fungal infections in animals.
万方数据
维普
