In vitro antifungal experimental study of "Xuanjing liniment" against Trichophyton rubrum
Objective To study the antifungal effect of alcohol extracts from 9 traditional Chinese medicines,including golden larch bark、Fructus cnidii、chaulmoogratree seed,etc on Trichophyton rubrum,and to explore their effects on fungal morphology and ultrastructure through scanning and transmission electron microscopy.Methods Extracting effective com-ponents of traditional Chinese medicine by ethanol reflux method;determination of the minimum inhibitory concentration (MIC) of 9 traditional Chinese medicines against Trichophyton rubrum using the 12 well plate solid drug based method;ob-serving the changes in surface morphology and intracellular ultrastructure of fungal cells before and after the action of single drug and compound drug using scanning electron microscopy and transmission electron microscopy.Results The antifungal experiment showed that the MICs of golden larch bark、Fructus cnidii、chaulmoogratree seed、dandelion、Coptis chinensis、dark plum、golden cypress、Kochia scoparia、Sophora flavescens against Trichophyton rubrum were 1.562 mg/mL,1.562 mg/mL,3.125 mg/mL,3.125 mg/mL,12.5 mg/mL,12.5 mg/mL,25 mg/mL,50 mg/mL,and>50 mg/mL,respec-tively;seven traditional Chinese medicines,including golden larch bark、Fructus cnidii、chaulmoogratree seed,etc,were se-lected to be a compound and conducted antifungal experiments,and the MIC result of the compound was 0.545 mg/mL;scanning electron microscopy showed that after 72 hours of treatment with Fructus cnidii,golden larch bark,and com-pound,the fungal hyphae and spores showed varying degrees of dissolution,breakage,and destruction;transmission elec-tron microscopy showed that after 72 hours of treatment with Fructus cnidii,golden larch bark,and compound,the fungal organelles showed varying degrees of damage and content loss.Conclusion The antifungal effect of traditional Chinese medi-cine compound on Trichophyton rubrum was stronger than any of single drug.Among the single drugs,golden larch bark and Fructus cnidii showed good antifungal effect.The morphology and ultrastructure of Trichophyton rubrum were obvi-ously damaged and changed after the action of golden larch bark、Fructus cnidii and the compound.The degrees of damage were as below:compound>golden larch bark>Fructus cnidii.
"Xuanjing liniment"Trichophyton rubrumantifungal experimentscanning electron microscopetransmission electron microscope
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