首页|Amplifying colorectal cancer progression:impact of a PDIA4/SP1 positive feedback loop by circPDIA4 sponging miR-9-5p

Amplifying colorectal cancer progression:impact of a PDIA4/SP1 positive feedback loop by circPDIA4 sponging miR-9-5p

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Objective:Colorectal cancer(CRC)is a prevalent malignant tumor with a high fatality rate.CircPDIA4 has been shown to have a vital role in cancer development by acting as a facilitator.Nevertheless,the impact of the circPDIA4/miR-9-5p/SP1 axis on development of CRC has not been studied.Methods:Western blot,immunohistochemistry,and reverse transcription-quantitative polymerase chain reaction assays were used to analyze gene expression.The CCK-8 assay was used to assess cell growth.The Transwell assay was used to detect invasion and migration of cells.The luciferase reporter and RNA immunoprecipitation tests were used to determine if miR-9-5p and circPDIA4(or SP1)bind to one another.An in vivo assay was used to measure tumor growth.Results:It was shown that circPDIA4 expression was greater in CRC cell lines and tissues than healthy cell lines and tissues.CircPDIA4 knockdown prevented the invasion,migration,and proliferation of cells in CRC.Additionally,the combination of circPDIA4 and miR-9-5p was confirmed,as well as miR-9-5p binding to SP1.Rescue experiments also showed that the circPDIA4/miR-9-5p/SP1 axis accelerated the development of CRC.In addition,SP1 combined with the promoter region of circPDIA4 and induced circPDIA4 transcription.CircPDIA4 was shown to facilitate tumor growth in an in vivo assay.Conclusions:The circPDIA4/miR-9-5p/SP1 feedback loop was shown to aggravate CRC progression.This finding suggests that the ceRNA axis may be a promising biomarker for CRC patient treatment.

CircRNApositive feedback loopcolorectal cancerPDIA4SP1

Yan Zhuang、Yiding Ai、Peng Li、Xin Yue、Yue Li、Luling Shan、Tongtong Wang、Peng Zhao、Xun Jin

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Department of Colorectal Oncology,Tianjin Medical University Cancer Institute&Hospital,National Clinical Research Center for Cancer,Tianjin's Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin 300060,China

Department of Biochemistry and Molecular Biology,Tianjin Medical University Cancer Institute&Hospital,National Clinical Research Center for Cancer,Tianjin's Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin 300060,China

Department of Neurosurgery and Neuro-Oncology,Tianjin Medical University Cancer Institute&Hospital,National Clinical Research Center for Cancer,Tianjin's Clinical Research Center for Cancer,Key Laboratory of Cancer Prevention and Therapy,Tianjin,Tianjin 300060,China

Department of Gastro Colorectal Oncology,Tianjin Cancer Hospital Airport Hospital,Tianjin 300308,China

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National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaScience and Technology Project of Tianjin Binhai New Area Health CommissionChina Digestive Tumor Clinical Scientific Research Public Welfare Project

82073276822731002022BWKY016P014-058

2024

10.20892/j.issn.2095-3941.2024.0112

癌症生物学与医学(英文版)
中国抗癌协会

癌症生物学与医学(英文版)

CSTPCD
影响因子：1.07
ISSN：2095-3941
年,卷(期)：2024.21(10)