目的:探讨X-box结合蛋白1(X-box binding protein 1，XBP1)及程序性死亡配体1(programmed death-ligand 1，PD-L1)在皮肤恶性黑色素瘤(cutaneous malignant melanoma，CMM)中的表达及其临床意义。方法:选取2017年1月至2023年7月宁夏医科大学总医院收治的53例CMM患者和56例色素痣患者，采用免疫组织化学染色法检测恶性黑色素瘤组织及色素痣组织XBP1及PD-L1表达水平，并分析XBP1及PD-L1表达水平与CMM患者临床特征的关系。应用Sperman分析XBP1及PD-L1在CMM中的相关性。结果:与色素痣组织相比，CMM组织XBP1及PD-L1的表达水平均显著升高，差异具有统计学意义(P<0。05)。CMM患者XBP1表达水平升高与临床分期Ⅱ～Ⅳ期、肿瘤浸润淋巴细胞(tumor-infiltrating lymphocytes，TILs)分级2～3级有关(P<0。05)，CMM患者PD-L1表达水平升高与原发灶溃疡、临床分期Ⅱ～Ⅳ期、Clark分级Ⅳ～Ⅴ级、TILs分级2～3级有关(P<0。05)。Sperman分析显示，XBP1及PD-L1在CMM中呈正相关(P<0。05)。结论:XBP1及PD-L1可作为CMM诊断及病情判断的分子标志物，且XBP1可能是通过调控PD-L1的表达，进而改变肿瘤微环境，影响CMM的发生发展。
Expression of XBP1 and PD-L1 in cutaneous malignant melanoma and their clinical significance
Objective:To investigate the expression of X-box binding protein 1 (XBP1) and programmed death-ligand 1 (PD-L1) in cutaneous malignant melanoma (CMM) and their clinical significance. Methods:Fifty-three patients with CMM and fifty-six patients with pigmented nevus were selected from those admitted to the General Hospital of Ningxia Medical University between January 2017 and July 2023. The expression levels of XBP1 and PD-L1 in malignant melanoma tissues and pigmented nevus tissues were detected using immunohistochemic-al staining. The relationships between the expression levels of XBP1 and PD-L1 and the clinical characteristics of patients with CMM were then analyzed. Spearman's analysis was applied to assess the correlation between XBP1 and PD-L1 expression levels in CMM. Results:The expression levels of XBP1 and PD-L1 were significantly higher in CMM tissues than those in pigmented nevus tissues (P<0.05). Elevated XBP1 expression levels in patients with CMM were associated with clinical stages Ⅱ-Ⅳ and tumor-infiltrating lymphocytes (TILs) grade 2-3 (P<0.05). Similarly,elevated PD-L1 expression levels in patients with CMM were associated with ulceration of the primary foci,clinical stages Ⅱ-Ⅳ,Clark grades Ⅳ-Ⅴ,and TILs grades 2-3 (P<0.05). Spearman's analysis demonstrated a positive correlation between XBP1 and PD-L1 expression in CMM (P<0.05). Conclusions:XBP1 and PD-L1 can be used as molecular markers for the diagnosis and evaluation of CMM. Ad-ditionally,XBP1 may affect the development of CMM by regulating the expression of PD-L1,thereby altering the tumor microenvironment.
万方数据
维普
