Effect of gemcitabine combined with TRAIL on proliferation and apoptosis of lung cancer NCI-H460 cells
Objective:To study the effect of gemcitabine combined with TRAIL (TNF-related apoptosis inducing ligand) on proliferation and apoptosis of human lung cancer NCI-H460 cells.Methods: MTT assay was used to analyze the proliferation of NCI-H460 cells, and flow cytometry was used to examine the apoptosis of NCI-H460 cells.Results: Gemcitabine or TRAIL alone or in combination inhibited the proliferation of NCI-H460 cells in a time-(24, 48, 72 h) and concentration-dependent (0.001 - 10 μg/ml) manner.Gemcitabine combined with TRAIL had a stronger inhibitory effect than they were used alone, with the inhibitory ratios being 14.9％ -77.5％ , 23.4％ -74.8％ , and 22.3％ -80.5％ after 72 h in combination, gemcitabine, and TRAIL groups, and the IC50 being 0.0853, 0.0982, and 0.0435 μg/ml respectively.The inhibitory effect of gemcitabine combined with TRAIL on proliferation of NCI-H460 cells was related to the administration order and the ratio of gemcitabine and TRAIL; gemcitabine treatment for 24 h and then giving TRAIL had a stronger inhibitory effect than TRAIL treatment for 24 h and then giving gemcitabine; when the ratio of gemcitabine and TRAIL was 1∶0.3, the combination showed the strongest inhibitory effect.The apoptosis rate of NCI-H460 cells in the combination treatment group was significantly higher than in the other two groups (49.04％ vs 29.33％ , 25.69％ , P ＜ 0.01 ).Conclusion: Gemcitabine and TRAIL have synergistic effects in inhibiting proliferation and promoting apoptosis of lung cancer NCI-H460 cells.
gemcitabineTNF-related apoptosis inducing ligand (TRAIL)human lung cancer cellNCI-H460proliferastionapoptosis
国家科技期刊平台
NSTL
万方数据
维普
