摘要
目的:探讨"肺肠同治"宣白承气汤对重症急性胰腺炎(SAP)早期急性肺损伤(ALI)的影响及作用.方法:将80只SD大鼠随机分为对照组、模型组、宣白承气汤组、宣白组和承气组,每组16只.除对照组外,其余各组以胰胆管注射牛磺胆酸钠的方式制备SAP模型,于造模后3、6、12、24h各时间点分别处死4只大鼠取材,观察大鼠胰腺、肺和肠组织的病理学表现及促炎因子IL-1β、IL-6和TNF-a水平.结果:宣白组和承气组的病理学改变相近,宣白承气汤组明显优于宣白组和承气组(P＜0.05).在各时间点,模型组较对照组IL-1β、IL-6和TNF-α水平明显升高,中药各组较模型组明显下降,宣白承气汤组优于宣白组和承气组(P＜0.05).除对照组外,其余各组IL-1β、IL-6和TNF-α水平在第12h达到高峰.结论:肠道在SAP-ALI发病及防治过程中起着关键作用,宣白承气汤可通过肺肠同治明显减轻SAP-ALI炎症水平,延缓疾病的发生发展.
Abstract
Objective:To investigate the effect of Xuanbaichengqi Decoction,which is a"lung and intestinal treatment",on early lung injury in severe acute pancreatitis.Methods:Eighty SD rats were randomly divided into control group,model group,Xuanbaichengqi Decoction group,Xuanbai group and Chengqi group,16 rats in each group.Except for the control group,the SAP model was prepared by pancreaticobiliary injection of sodium tauro-glycocholate in each group,and four rats were executed at each time point of 3,6,12,and 24 h after modeling to obtain the materials,and the pathological manifestations of the pancreas,lung,and intestinal tissues of the rats and the levels of the pro-inflammatory factors IL-1β,IL-6,and TNF-α were observed.Results:The pathological changes were similar in the Xuanbai and Chengqi groups,and the Xuanbaichengqi Decoction group was significant-ly better than the Xuanbai and Chengqi groups(P＜0.05).At all time points,the levels of IL-1β,IL-6 and TNF-α were significantly higher in the model group than in the control group,and significantly lower in the traditional Chinese medicine groups than in the model group,and the Xuanbaichengqi Decoction group was superior to the Xuanbai and Chengqi groups(P＜0.05).Except for the control group,the levels of IL-1β,IL-6 and TNF-α in the remaining groups reached the peak at the 12 h.Conclusion:The intestinal tract plays a key role in the pathogenesis and prevention of SAP-ALI,and Xuanbaichengqi Decoction can significantly reduce the inflammation level of SAP-ALI and delay the development of the disease through the treatment of lung and intestines together.
基金项目
天津市卫生健康委中医中西医结合科研项目(2019075)
万方数据