Mechanistic study of the therapeutic effect of berberine on ulcerative colitis in mice based on the Bak/Bax/Caspase-9 pathway
Objective:This study aimed to investigate the protective effect of berberine on mice with ulcerative colitis(UC)and the involvement of the Bak/Bax/Caspase-9 pathway.Methods:A total of thirty-two C57BL/6 mice were divided into four groups:normal group,model group,mesalazine group,and berberine group.The mice were randomly assigned to these groups for the study.The UC mouse model was induced in all groups using 3％dextran sulfate sodium salt(DSS).Disease activity index(DAI)score and body weight were recorded daily.Colon length and spleen index were measured.HE staining examined colon and spleen tissue changes.Bak,Bax,and Caspase-9 mRNA/protein expression in colon was measured via qRT-PCR/Western blot.Results:Compared to the model group,the berberine group exhibited a significant reduction in the DAI score and spleen index(P＜0.05),along with improved colon and spleen pathological damage,there were significant increases in percentage of body weight change and colon length(P＜0.01),mRNA and protein expression of Bak,Bax,and Caspase-9 in co-lon tissues were significantly decreased in the berberine group(P＜0.05).Conclusion:Berberine reduced inflam-mation and improved colon and spleen pathological damage in UC mice,potentially through modulation of the Bak/Bax/Caspase-9 pathway.
万方数据
维普
