Mechanistic study of the therapeutic effect of Portulaca oleracea L on colorectal inflammatory cancer transformation in mice by inhibiting Caspase 12 protein based on proteomics
Objective:To investigate the mechanism of the protective effect of Portulaca oleracea L on colorec-tal inflammatory cancer transformation model mice through the NOD signaling pathway mediated by Caspase 12.Methods:Twenty-four C57 mice aged 6-8 W were randomly divided into normal control,model control,and por-tulaca oleracea L treatment groups.In the model control group and portulaca oleracea L treatment group,Azoxy-methane(AOM)combined with Dextran sodium sulfate(DSS)was used to induce colitis-associated tumorigenesis mice model,and in the portulaca oleracea L treatment group,gavage the drug every day according to the equiva-lent dose of human mice.The efficacy of Portulaca oleracea L in inhibiting colorectal cancer transformation mice was analyzed by ratio of spleen/body,colorectal length,total number of polyps/tumors,and HE staining of intes-tinal tissues in each group.Proteomic analysis and Western blot were used to clarify the therapeutic effect of the NOD signaling pathway,especially the key protein Caspase 12.The expression of cytokine IL-1β and macrophage marker F4/80 in mice tissues were detected by qPCR and immunofluorescence,respectively.Finally,the level of intestinal mucin was detected by Alican blue and PAS pathological staining,and the level of intestinal tight junc-tion protein was detected by Western blot,to evaluate the protective effect of Portulaca oleracea L on the intestinal barrier.Results:Portulaca oleracea L significantly inhibited the progression of colorectal inflammatory cancer transformation in mice,and reduced the number of intestinal polyps/tumors and intestinal Ki67 levels.Portulaca oleracea L significantly improved the function of the NOD-like receptor signaling pathway in colorectal inflamma-tory cancer transformation mice,inhibited the level of the key protein Caspase 12,reduced the levels of the down-stream cytokine IL-1β and chemokine MCP-1,and inhibited the expression of macrophages in the intestinal tis-sues.Portulaca oleracea L improved the intestinal barrier function in colorectal inflammation cancer-transformed mice.Conclusion:Portulaca oleracea L significantly inhibited the progression of colorectal inflammatory cancer transformation in mice models,suppressed inflammatory responses,and improved the intestinal barrier,and its effects may be related to the modulation of the Caspase 12-mediated NOD-like receptor signaling pathway.
colorectal inflammatory cancer transformationPortulaca oleracea LCaspase 12intestinal barrier
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