The study of N-methyl-N-nitrosourea combined with Helicobacter pylori infection inducing gastric inflammation-cancer transformation process in mice
Objective:To investigate the role of N-methyl-N-nitrosourea(MNU)combined with Helicobacter pylori(Hp)in the"inflammation-cancer"transformation process of gastric mucosa in C57BL/6J mice.Methods:A total of 112 C57BL/6J mice were divided into a control group(3,6,9,12 months,n=8)and a model group(3,6,9,12 months,n=20).Mice in the model group were molded by MNU combined with Hp infection.Tissues were collected from the mice in the modeling group at 3,6,9,and 12 months respectively,to observe tumor for-mation in gastric mucosa and the impact of this modeling method on the transcription levels of gastric inflamma-tion factors.Results:The tumor formation rates in the 9-month and 12-month groups of MNU combined with Hp modeling were approximately 30％and 40％,respectively.After modeling for 9 and 12 months,extensive infil-tration of inflammatory cells,mainly lymphocytes and eosinophils,was observed in the gastric mucosa,accompa-nied by dysplastic changes.The mRNA levels of TNF-α showed a significant upregulation in the 6,9,and 12-month groups after infection.IL-1β was significantly upregulated in the 3,and 6-month groups and declined in the 12-month group.The expression of IL-33,associated with precancerous lesions of gastric cancer,did not show significant upregulation in this study but was significantly downregulated in the 12-month model group.Conclu-sion:MNU combined with Hp modeling showed significant manifestations of inflammation infiltration,with tumor formation rates of approximately 30％and 40％in the 9-month and 12-month groups,respectively.This combined modeling can accelerate the pathological changes from chronic gastritis to dysplasia in C57BL/6J mouse gastric mucosa.
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