The effect of Schisandrin A on miR-873-5p/G6PD axis regulation on the viability,apoptosis,and aerobic glycolysis of gastric cancer SGC-7901 cells
Objective:To investigate the effect of Schisandrin A on the regulation of microRNA-873-5p(miR-873-5p)/glucose-6-phosphate dehydrogenase(G6PD)axis on the viability,apoptosis,and aerobic glycolysis of gastric cancer SGC-7901 cells.Methods:Divide gastric cancer SGC-7901 cells into SGC-7901 group,low concen-tration Schisandrin A group,high concentration Schisandrin A group,high concentration Schisandrin A+empty plasmid group,and high concentration Schisandrin A+miR-873-5p silencing group.Each group was given corre-sponding drug intervention and transfection,and cultured for 48 hours.Cell viability and apoptosis rate were de-tected using cell count kit-8 and flow cytometry,respectively;the reagent kit detects glucose consumption,lactate production,and adenosine triphosphate(ATP)production;real-time fluorescence quantitative PCR was used to detect the expression of miR-873-5p and G6PD mRNA;western blot was used to detect the expression of G6PD and apoptosis-related proteins;targeting relationship between miR-873-5p and G6PD detected by dual luciferase reporter gene detection.Results:Compared with SGC-7901 group,the cell viability,B cell lymphoma-2(Bcl-2)protein,glucose consumption,lactate production,ATP production relative levels,G6PD mRNA and protein lev-els in low and high concentration Schisandrin A groups were significantly decreased,apoptosis rate,Bcl-2 related X protein(Bax),cleaved-Caspase-3 protein and miR-873-5p levels were significantly increased(P＜0.05);The cell viability,Bcl-2 protein,glucose consumption,lactate production,ATP production relative levels,G6PD mRNA and protein levels in the high concentration Schisandrin A group were significantly lower than those in the low con-centration Schisandrin A group,the cell apoptosis rate,Bax,cleaved-Caspase-3 protein,miR-873-5p levels were significantly higher than those in the low concentration Schisandrin A group(P＜0.05);Compared with high con-centration Schisandrin A group and high concentration Schisandrin A+empty plasmid group,the cell viability,Bcl-2 protein,glucose consumption,lactate production,ATP production relative levels,G6PD mRNA and protein levels in the high concentration Schisandrin A+miR-873-5p silencing group were significantly increased,the cell apoptosis rate,Bax,cleaved-Caspase-3 protein,miR-873-5p levels were significantly decreased(P＜0.05);miR-873-5p can target and regulate the expression of G6PD.Conclusion:Schisandrin A can target and downregulate G6PD expression by upregulating miR-873-5p,thereby inhibiting the viability and aerobic glycolysis of gastric cancer SGC-7901 cells,and promoting their apoptosis.
Schisandrin AmicroRNA-873-5pglucose-6-phosphate dehydrogenasegastric cancer SGC-7901 cellscell viabilityapoptosisaerobic glycolysis
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